Datta S, Kankel D R
Department of Biology, Yale University, New Haven, Connecticut 06511-8112.
Genetics. 1992 Mar;130(3):523-37. doi: 10.1093/genetics/130.3.523.
Adult optic lobes of Drosophila melanogaster are composed of neurons specific to the adult which develop postembryonically. The structure of the optic lobes and aspects of its development have been described, and a number of mutants that affect its development have been identified. The focus of every screen to date has been on disruption of adult structure or function. Although these loci were originally identified on the basis of viable mutants, some have proven capable of giving rise to lethal alleles. It seems reasonable to assume that mutants which strongly affect development of the imaginal-specific central nervous system may evidence abnormalities during the late larval or pupal stages when the adult central nervous system is undergoing final assembly and might show a lethal phase prior to eclosion (as is true for mutations at the previously defined l(1)ogre locus). We have carried out the first screen of autosomal and sex-linked late larval and pupal lethals to identify mutations that affect the development of the optic lobes. Our screen yielded nine mutants that could tentatively be grouped into three classes, depending on the neuroblast population affected and imaginal disc phenotypes. Two of these, including one that is allelic to l(1)zw1, were chosen for further analysis.
黑腹果蝇的成虫视叶由成虫特有的神经元组成，这些神经元在胚胎后期发育。视叶的结构及其发育方面已被描述，并且已经鉴定出一些影响其发育的突变体。迄今为止，每个筛选的重点都在于破坏成虫的结构或功能。尽管这些基因座最初是基于存活突变体鉴定出来的，但有些已被证明能够产生致死等位基因。似乎有理由假设，强烈影响成虫特异性中枢神经系统发育的突变体，可能在幼虫后期或蛹期出现异常，此时成虫中枢神经系统正在进行最终组装，并且可能在羽化前呈现致死阶段（就先前定义的l(1)ogre基因座的突变而言就是如此）。我们对常染色体和性连锁的晚期幼虫和蛹期致死突变体进行了首次筛选，以鉴定影响视叶发育的突变。我们的筛选产生了九个突变体，根据受影响的神经母细胞群体和成虫盘表型，这些突变体可以初步分为三类。其中两个，包括一个与l(1)zw1等位的突变体，被选作进一步分析。
