Kim Sun-Uk, Han Ying-Hao, Lee Tae-Hoon, Hyun Byung-Hwa, Lee Sang-Ho, Lee Dong-Seok, Yu Dae-Yeul
Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Exp Anim. 2004 Oct;53(5):475-7. doi: 10.1538/expanim.53.475.
Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.
对交配后2.5天或3.5天获得的C57BL/6小鼠囊胚进行过夜体外培养后，检查其进行有效显微注射的情况。自然交配后3.5天（每只小鼠1.05个）和超排卵后3.5天（每只小鼠2.83个）无透明带胚胎的发生率远高于自然交配后2.5天（每只小鼠0.05个）和超排卵后2.5天（每只小鼠0.01个）。通过测试基因靶向J1胚胎干细胞的种系能力，应推荐采用超排卵和/或体外培养来生产用于产生高种系嵌合体的可显微注射囊胚。
