Korbutt G S, Mallett A G, Ao Z, Flashner M, Rajotte R V
Surgical-Medical Research Institute, Dentistry/Pharmacy Building, University of Alberta, Edmonton, Alberta, Canada.
Diabetologia. 2004 Oct;47(10):1810-8. doi: 10.1007/s00125-004-1531-3. Epub 2004 Oct 23.
AIMS/HYPOTHESIS: The aim of this study was to determine whether a simple alginate capsule can prolong islet survival and function during long-term tissue culture. We also wanted to observe the ability of these encapsulated islets to restore glucose responsiveness to diabetic recipients, along with the quantity of islets required to do so.
We compared the recovery and metabolic function of encapsulated canine islets with that of non-encapsulated canine islets following 1, 2 or 3 weeks of tissue culture. These culture preparations were also transplanted into diabetic nude mice and compared for their ability to reverse diabetes. Furthermore, short-term cultured encapsulated and non-encapsulated islets were transplanted in varying numbers to determine the minimum dose required to normalise blood glucose and prolong recipient survival.
Islet recovery following 1, 2 and 3 weeks of tissue culture was significantly higher when islets were encapsulated. When these islets were recovered at 1, 2 and 3 weeks and transplanted into diabetic nude mice, survival at 100 days was 100% for all encapsulated groups, versus 66%, 33% and 33% respectively for the non-encapsulated islets. Additionally, substantially fewer short-term cultured islets were required to normalise blood glucose when the islets were encapsulated. Recipients of encapsulated islets also had significantly longer survival times than recipients of non-encapsulated preparations.
CONCLUSIONS/INTERPRETATION: This study demonstrates that encapsulation of islets with purified alginate improves islet survival and function in vitro and in vivo.
目的/假设：本研究的目的是确定一个简单的藻酸盐胶囊能否在长期组织培养过程中延长胰岛的存活时间并维持其功能。我们还想观察这些封装胰岛恢复糖尿病受体葡萄糖反应性的能力，以及实现这一目标所需的胰岛数量。
我们比较了组织培养1、2或3周后封装犬胰岛与未封装犬胰岛的恢复情况和代谢功能。这些培养制剂还被移植到糖尿病裸鼠体内，并比较它们逆转糖尿病的能力。此外，将短期培养的封装和未封装胰岛以不同数量进行移植，以确定使血糖正常化并延长受体存活时间所需的最小剂量。
组织培养1、2和3周后，封装胰岛的恢复率显著更高。当这些胰岛在1、2和3周后恢复并移植到糖尿病裸鼠体内时，所有封装组在100天时的存活率为100%，而未封装胰岛组的存活率分别为66%、33%和33%。此外，封装胰岛使血糖正常化所需的短期培养胰岛数量大幅减少。封装胰岛受体的存活时间也显著长于未封装制剂的受体。
结论/解读：本研究表明，用纯化藻酸盐封装胰岛可改善其在体外和体内的存活及功能。
