Jakucs Erzsébet, Kovács G M, Agerer R, Romsics C, Eros-Honti Z
Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/c, 1117 , Budapest, Hungary.
Mycorrhiza. 2005 Jun;15(4):247-58. doi: 10.1007/s00572-004-0326-1. Epub 2004 Oct 28.
Species in the genus Tomentella (Thelephoraceae) belong to the most frequent and widespread ectomycorrhizal (EM) fungi found in temperate and boreal forests. Although several unidentified tomentelloid morphotypes have been presented as common members of EM communities in coniferous and broad-leaved forests, few tomentelloid EM have been identified and described in detail. In this study, ten tomentelloid EM isolates collected from Populus alba, Quercus cerris and Picea abies stands in Hungary and Germany are characterized and documented by morphological-anatomical methods using light microscopy. The investigated ectomycorrhizae belong to the same brown-black tomentelloid morphotype but form two different anatomotype groups (At I and At II). Molecular taxonomical identification was accomplished using phylogenetic analysis (neighbor joining method) of 49 Tomentella nrDNA-ITS nucleotide sequences including the 10 new and 39 GenBank sequences. The EM isolates clustered into two adjoining clades identical with the two anatomotypes. At II clustered with Tomentella stuposa while At I could not be identified to species. Based on the morphological similarity and the low genetic difference it must be a closely related taxon. A comparison of the recently known tomentelloid EM to T. stuposa is presented. Ecological questions involving abundance and host relationships are discussed.
绒毛革菌属（革菌科）的物种是温带和北方森林中最常见且分布广泛的外生菌根（EM）真菌。尽管有几种未鉴定的绒毛革菌形态型被认为是针叶林和阔叶林中外生菌根群落的常见成员，但很少有绒毛革菌外生菌根被详细鉴定和描述。在本研究中，利用光学显微镜通过形态解剖学方法对从匈牙利和德国的银白杨、栓皮栎和欧洲云杉林分中采集的10个绒毛革菌外生菌根分离物进行了特征描述和记录。所研究的外生菌根属于同一棕黑色绒毛革菌形态型，但形成了两个不同的解剖型组（At I和At II）。使用系统发育分析（邻接法）对49个绒毛革菌nrDNA-ITS核苷酸序列进行分子分类鉴定，其中包括10个新序列和39个GenBank序列。外生菌根分离物聚为两个相邻的进化枝，与两个解剖型相同。At II与致密绒毛革菌聚在一起，而At I无法鉴定到种。基于形态相似性和低遗传差异，它一定是一个密切相关的分类群。本文对最近已知的绒毛革菌外生菌根与致密绒毛革菌进行了比较。讨论了涉及丰度和宿主关系的生态问题。
