Jakucs Erzsébet, Kovács Gábor M, Szedlay Gyöngyi, Eros-Honti Zsolt
Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/c, 1117 Budapest, Hungary.
Mycorrhiza. 2005 Sep;15(6):459-70. doi: 10.1007/s00572-005-0351-8. Epub 2005 Mar 3.
The most common representatives of tomentelloid ectomycorrhizae (EM) collected in broad-leaved forests (Populus and Quercus) of the Hungarian Plain during a 4-year project are demonstrated. Eighteen specimens of nine tomentelloid EM morphotypes were investigated. Five of these, introduced here for the first time, were characterized by microscopical-morphological and anatomical methods. Molecular identification was carried out using sequence analysis of the nrDNA ITS region. Altogether, 54 ITS sequences (6 previously published and 12 new sequences from our mycorrhizae and 36 from GenBank derived from fruitbodies) were compared by phylogenetic analyses using neighbor-joining, maximum parsimony and maximum likelihood methods. Identification to species level was successful in the case of six EM morphotypes (Tomentella galzinii, T. subtestacea, T. sublilacina, T. pilosa, T. ferruginea and T. stuposa), and the possible taxonomic position of the remaining three was approached. These results are supported by morphology, as compared with literature data. Relative abundance of the EM morphotypes within the soil samples was estimated. Our results confirm that tomentelloid EM are constant, diverse and abundant members of the EM communities in temperate-continental broad-leaved forests.
本文展示了在匈牙利平原阔叶林（杨树和栎树）为期4年的项目中收集到的绒毛状外生菌根（EM）的最常见代表类型。对9种绒毛状EM形态类型的18个标本进行了研究。其中5种形态类型首次在此介绍，通过微观形态学和解剖学方法对其进行了特征描述。利用nrDNA ITS区域的序列分析进行分子鉴定。通过邻接法、最大简约法和最大似然法进行系统发育分析，总共比较了54个ITS序列（6个先前已发表，12个来自我们菌根的新序列，36个来自GenBank中源自子实体的序列）。6种EM形态类型（绒毛绒盖牛肝菌、亚浅黄绒盖牛肝菌、亚淡紫绒盖牛肝菌、绒毛丝膜菌、铁锈色丝膜菌和瘤状丝膜菌）成功鉴定到种水平，并探讨了其余3种的可能分类地位。与文献数据相比，这些结果得到了形态学的支持。估计了土壤样本中EM形态类型的相对丰度。我们的结果证实，绒毛状EM是温带大陆阔叶林EM群落中稳定、多样且丰富的成员。
