Buda Andrea, Sands Caroline, Jepson Mark A
Department of Surgical and Gastroenterological Sciences, University of Padua, 35010 Padua, Italy.
Adv Drug Deliv Rev. 2005 Jan 2;57(1):123-34. doi: 10.1016/j.addr.2004.07.014.
Fluorescence imaging technology can be applied to many aspects of cell biology ranging from the analysis of specific markers in cells and tissues to the biological actions and distribution of fluorescent proteins or particles in living cells. In this review, we examine the role of fluorescence imaging, in conjunction with other microscopical techniques, to study sites of uptake of material across the gastrointestinal epithelium. We will focus primarily on intestinal M cells, specialised antigen-sampling cells in the epithelium of the gut-associated lymphoid tissue (GALT), including Peyer's patches. In addition to their importance as sites for uptake of inert material, and hence their potential as a route of delivery of vaccines, etc., M cells are also a major site of infection by a range of microbial pathogens. The application of new fluorescence imaging technologies has expanded our knowledge on the structure, development and function of these fascinating cells.
荧光成像技术可应用于细胞生物学的许多方面，从细胞和组织中特定标志物的分析到荧光蛋白或颗粒在活细胞中的生物学作用和分布。在这篇综述中，我们研究了荧光成像与其他显微镜技术相结合在研究物质跨胃肠上皮摄取部位方面的作用。我们将主要关注肠道M细胞，它是肠道相关淋巴组织（GALT）上皮中的特殊抗原采样细胞，包括派尔集合淋巴结。除了作为惰性物质摄取部位的重要性以及因此作为疫苗等递送途径的潜力外，M细胞也是一系列微生物病原体的主要感染部位。新荧光成像技术的应用扩展了我们对这些迷人细胞的结构、发育和功能的认识。
