Wan Deguang, Pei Jin, Yan Zhuyun
College of Pharmacy, Chengdu University of TCM, Chengdu 610075.
Zhong Yao Cai. 2004 Jun;27(6):397-8.
To compare the content of hyperin in Hypericum medicinal plants.
HPLC method was established. Alltima C18 radial compression column was used with a mobile phase of methanol-0.5% phosphoric acid (45:55, adjusted to pH = 3.0 with triethylamine). The detecting wavelength was 360 nm. Flow rate was 0.8 ml/min, external standard method was quantitative analysis method.
The linear range was 0.101-2.023 microg. The average recovery was 96.527%. The result showed that the content of hyperin in H. perforatum, H. forrestii was higher than that of others (1.257% and 0.461% respectively).
This method provides an accurate and sensitive way in detecting hyperin.
比较金丝桃属药用植物中金丝桃苷的含量。
建立高效液相色谱法。采用Alltima C18径向压缩柱，以甲醇 - 0.5%磷酸（45:55，用三乙胺调至pH = 3.0）为流动相。检测波长为360 nm。流速为0.8 ml/min，采用外标法进行定量分析。
线性范围为0.101 - 2.023 μg。平均回收率为96.527%。结果表明，贯叶金丝桃、川滇金丝桃中金丝桃苷的含量高于其他植物（分别为1.257%和0.461%）。
该方法为检测金丝桃苷提供了一种准确、灵敏的方法。
