Chen Mei-lan, Huang Lu-qi, Yang Bin, Fu Mei-hong, Luo Yong-ming
Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700.
Zhongguo Zhong Yao Za Zhi. 2003 Nov;28(11):1013-5.
To establish the method of determining the quantity of hypericin in Hypericum perforatum and determine the quantity of the hypericin in defferent medicinal materials and asepsis seedings which grow in defferent environment.
The specimen is extracted with methanol--Pyridine (9:1) ultrasound extraction. Chromatographic assay is performed on a hypersily ODS2 (4.6 mm x 150 mm, 5 microm) column. The mobile phase is composed of methanol -1.56% dihydric natrium phosphate hydrogen natrium solution (shift solution's acidity to 2.1 with phosphoric acid)--ethyl acetate (4:1.9:1), velocity of flow is 1 mL x min(-1); column temperature is 35 degrees C; the detection wavelength is 590 nm.
A satisfactory seperaration between hypericin and impurity. The calibration curve is linear over the range of 0.0524-0.2620 microg for hypericin (r = 0.9998). The average recovery of hypericin is 97.50%.
The quantity of hypericin in Hypericum perforatum has something to do with the genetic factor, environment factor, growing period and dry means. The method of determining the quantity of hypericin can be regarded as the method of controling the quantity of medicinal materials.
建立贯叶连翘中金丝桃素含量测定方法，并测定不同产地药材及不同环境下无菌苗中金丝桃素的含量。
样品采用甲醇 - 吡啶（9:1）超声提取。色谱分析在Hypersily ODS2（4.6 mm×150 mm，5 µm）柱上进行。流动相由甲醇 - 1.56%磷酸二氢钠 - 磷酸氢二钠溶液（用磷酸调溶液酸度至2.1） - 乙酸乙酯（4:1.9:1）组成，流速为1 mL·min⁻¹；柱温为35℃；检测波长为590 nm。
金丝桃素与杂质分离良好。金丝桃素在0.0524 - 0.2620 µg范围内校准曲线呈线性（r = 0.9998）。金丝桃素的平均回收率为97.50%。
贯叶连翘中金丝桃素的含量与遗传因素、环境因素、生长时期及干燥方式有关。金丝桃素含量测定方法可作为药材质量控制方法。
