Kusznierz G F, Latini O A, Sequeira M D
Instituto Nacional de Enfermedades Respiratorias, Santa Fe, Argentina.
Int J Tuberc Lung Dis. 2004 Oct;8(10):1234-41.
Tuberculosis Laboratory Network, Argentina.
Registers of technical evaluation results for AFB microscopy were analysed. The quality of specimens, smears, staining and readings were evaluated, as was the relationship between these parameters.
The proportion of good quality specimens was considered acceptable. A direct relationship was demonstrated between positivity in results and mucopurulent sputum. The proportion of thin smears was relatively high. Positivity and bacillary count were lower in thin smears. Staining quality was considered good. The average agreement in readings throughout the country was 98%. Nevertheless, the false-positive rate was considered significant, and 46% of false-positive results were associated with defective staining.
The technical quality and agreement in the laboratory network were satisfactory. Nevertheless, improvements need to be made in the following: the quality of the smears, staining and reading, coverage, decentralisation of supervision, the slide selection method and data registration. Operational research on the storage and preservation of slides is also necessary.
阿根廷结核病实验室网络。
1）评估1983 - 2001年期间受监督的抗酸杆菌涂片显微镜检查的技术质量，以及2）分析操作误差对结果的影响。
分析抗酸杆菌显微镜检查技术评估结果的登记资料。对标本、涂片、染色和读数的质量以及这些参数之间的关系进行评估。
优质标本的比例被认为是可接受的。结果阳性与黏液脓性痰之间存在直接关系。薄涂片的比例相对较高。薄涂片中的阳性率和杆菌计数较低。染色质量被认为良好。全国读数的平均一致性为98%。然而，假阳性率被认为较高，46%的假阳性结果与染色缺陷有关。
实验室网络的技术质量和一致性令人满意。然而，在以下方面仍需改进：涂片、染色和读数的质量、覆盖范围、监督的分散化、玻片选择方法和数据登记。对玻片的储存和保存进行运筹学研究也是必要的。
