Dai Mu-Shui, Sun Xiao-Xin, Qin Jun, Smolik Sarah M, Lu Hua
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA.
Gene. 2004 Nov 10;342(1):49-56. doi: 10.1016/j.gene.2004.07.043.
Glutathione SH-transferase (GST) is a 25-kDa protein and a member of a large family that plays a critical role in the cellular homeostasis of all organisms. In this report, we describe a novel GST-containing protein identified and cloned from Drosophila. This 1045 amino acid protein possesses a zinc finger domain with a tandem array of four FLYWCH zinc finger motifs at its N-terminus and a C-terminal domain that shares a 46% homology with GST. The gene maps to chromosome 3 at position 84C6. Further characterization of this protein shows that it localizes to the cytoplasm of fly cells and is expressed through all stages of fly embryonic development. It binds to glutathione-S agarose beads in vitro. These results indicate that this new protein belongs to the GST family, thus named a Drosophila GST-containing FLYWCH zinc finger protein (dGFZF).
谷胱甘肽S-转移酶（GST）是一种25千道尔顿的蛋白质，是一个大家族的成员，在所有生物体的细胞内稳态中起关键作用。在本报告中，我们描述了一种从果蝇中鉴定并克隆的新型含GST的蛋白质。这种1045个氨基酸的蛋白质在其N端具有一个锌指结构域，带有四个FLYWCH锌指基序的串联阵列，以及一个与GST具有46%同源性的C端结构域。该基因定位于3号染色体上84C6的位置。对该蛋白质的进一步表征表明，它定位于果蝇细胞的细胞质中，并在果蝇胚胎发育的所有阶段都有表达。它在体外与谷胱甘肽-S琼脂糖珠结合。这些结果表明，这种新蛋白质属于GST家族，因此被命名为果蝇含GST的FLYWCH锌指蛋白（dGFZF）。
