静脉注射全氟化合物乳剂六个月后大鼠肝脏和肾脏的生化及形态学观察。

Biochemical and morphological observations on rat liver and kidneys six months after intravenous injection of a perfluorocompound emulsion.

作者信息

Branca D, Chiarelli S M, Roberti M S, Tortorella C, Antonini C, Vincenti E, Scutari G

机构信息

Department of Biological Chemistry, University of Padova, Italy.

出版信息

Life Sci. 1992;50(16):1157-63. doi: 10.1016/0024-3205(92)90458-2.

DOI:10.1016/0024-3205(92)90458-2

PMID:1552832

Abstract

The histological appearance of liver and kidneys and the energy metabolism of isolated liver and kidney mitochondria were evaluated in rats 6 months after intravenous administration of 1 ml of a perfluorocompound emulsion. Both liver and kidney specimens showed neither significant histological alteration nor the presence of intracytoplasmic perfluorocompound particles. A substantial depression of the rate of ATP synthesis was observed both in liver and kidney isolated mitochondria (with respect to control mitochondria) although the magnitude of the transmembrane electrical potential was unaltered. The depression of ATP synthesis in mitochondria isolated from perfluorocompound-treated rats appeared then unrelated to the presence of perfluorocompound micelles within the cells, and might result from the interaction of either the perfluorocompound or the emulsifying agent with the mitochondrial ATP synthetase.

摘要

在大鼠静脉注射1 ml全氟化合物乳剂6个月后，对其肝脏和肾脏的组织学外观以及分离的肝脏和肾脏线粒体的能量代谢进行了评估。肝脏和肾脏标本均未显示出明显的组织学改变，也未发现胞质内全氟化合物颗粒。尽管跨膜电位大小未改变，但在分离的肝脏和肾脏线粒体中均观察到ATP合成速率显著降低（相对于对照线粒体）。从全氟化合物处理的大鼠中分离出的线粒体中ATP合成的降低似乎与细胞内全氟化合物胶束的存在无关，可能是由于全氟化合物或乳化剂与线粒体ATP合成酶相互作用所致。