D'Souza Caroline, Prithvisagar Kattapuni Suresh, Deekshit Vijay Kumar, Karunasagar Indrani, Karunasagar Iddya, Kumar Ballamoole Krishna
Division of Infectious Diseases, Nitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangalore 575018, India.
Nitte (Deemed to be University), University Enclave, Medical Sciences Complex, Deralakatte, Mangaluru 575018, India.
Microorganisms. 2020 Jul 4;8(7):999. doi: 10.3390/microorganisms8070999.
It has been observed that not all strains of are virulent. Determining the virulence of strains that are frequently present in seafood is of significance for ensuring seafood safety. This study is an attempt to predict the virulence of seafood-borne isolated along the Mangaluru Coast, India. The isolates tested possessed a gene sequence with high similarity to that in the clinical strain. Transcriptional analysis of core virulence genes in seafood isolate E4010 showed the phenomenon of contact-mediated expression of which correlated well with the actin disintegration and cytotoxicity. These results suggest that the seafood isolates tested in this study possess a functional RtxA1 which could help in initiating the infection. However, other putative virulence genes such as encoding an extracellular protease, encoding hemolysin, encoding tad pilin and encoding fibronectin-binding protein were also constitutively expressed. Virulence-associated attributes such as cytotoxicity and adherence matched the response of the clinical strain ( > 0.05). On the other hand, the environmental strains showed higher serum sensitivity compared with the clinical strain. These findings show that the part of virulence attributes required for the disease process might be intact in these isolates.
据观察，并非所有的[具体菌株名称未给出]菌株都具有毒性。确定海鲜中常见菌株的毒性对于确保海鲜安全具有重要意义。本研究旨在预测印度芒格洛尔海岸分离出的海鲜源[具体菌株名称未给出]的毒性。所测试的分离株具有与临床菌株中高度相似的[基因名称未给出]基因序列。对海鲜分离株E4010中核心毒力基因的转录分析显示了[具体基因名称未给出]接触介导表达的现象，这与肌动蛋白解体和细胞毒性密切相关。这些结果表明，本研究中测试的海鲜分离株具有功能性的RtxA1，这可能有助于引发感染。然而，其他假定的毒力基因，如编码细胞外蛋白酶的[基因名称未给出]、编码溶血素的[基因名称未给出]、编码tad菌毛蛋白的[基因名称未给出]和编码纤连蛋白结合蛋白的[基因名称未给出]也组成性表达。细胞毒性和黏附等与毒力相关的特性与临床菌株的反应相符（P>0.05）。另一方面，环境菌株与临床菌株相比表现出更高的血清敏感性。这些发现表明，这些分离株中疾病过程所需的部分毒力特性可能是完整的。
