First Multi-Year Retrospective Study on and Prevalence in Harvested in Sacca Di Goro, Italy.

The present work describes a retrospective study aiming to verify a possible correlation between the environmental conditions (temperature, salinity and dissolved oxygen), the abundance of spp., and the prevalence of and in the Manila clam harvested in Sacca di Goro, Emilia-Romagna Region, Northern Italy. On the whole, 104 samples, collected in the period 2007-2015 and submitted to microbiological analyses (isolation and genotyping), have been reconsidered for spp. load, prevalence (total, gene marker toxRP; potentially pathogenic, gene markers and/or and prevalence (total, gene markers A and together with environmental data obtained from the monitoring activity of the Emilia-Romagna Regional Agency for the Prevention, the Environment and the Energy. Environmental data have been processed to calculate the median of each, assessing the seasonal range of seawater temperature (warmer months: April-October, T°C >16.45°C; cooler months November-March, T°C <16.45°C), salinity (27 psu), and dissolved oxygen (< or >8.2 mg/L). Total , total and potentially pathogenic were present respectively in the 11.5, 29.8 and 6.7% of the samples. The spp. load (mean value of 4.69±0.65 log colony forming unit g) and the prevalence of potentially pathogenic , were not significantly correlated to the environmental conditions (P>0.05), whereas the prevalence of both total and total was significantly higher in the warmer period (P<0.05), without correlation with salinity and dissolved oxygen values (P>0.05).