Boekhorst Jos, Siezen Roland J, Zwahlen Marie-Camille, Vilanova David, Pridmore Raymond D, Mercenier Annick, Kleerebezem Michiel, de Vos Willem M, Brüssow Harald, Desiere Frank
Center for Molecular and Biomolecular Informatics, 6525ED, Nijmegen, The Netherlands.
NIZO food research, 6710 BA Ede, The Netherlands.
Microbiology (Reading). 2004 Nov;150(Pt 11):3601-3611. doi: 10.1099/mic.0.27392-0.
The first comprehensive comparative analysis of lactobacilli was done by comparing the genomes of Lactobacillus plantarum (3.3 Mb) and Lactobacillus johnsonii (2.0 Mb). L. johnsonii is predominantly found in the gastrointestinal tract, while L. plantarum is also found on plants and plant-derived material, and is used in a variety of industrial fermentations. The L. plantarum and L. johnsonii chromosomes have only 28 regions with conservation of gene order, totalling about 0.75 Mb; these regions are not co-linear, indicating major chromosomal rearrangements. Metabolic reconstruction indicates many differences between L. johnsonii and L. plantarum: numerous enzymes involved in sugar metabolism and in biosynthesis of amino acids, nucleotides, fatty acids and cofactors are lacking in L. johnsonii. Major differences were seen in the number and types of putative extracellular proteins, which are of interest because of their possible role in host-microbe interactions. The differences between L. plantarum and L. johnsonii, both in genome organization and gene content, are exceptionally large for two bacteria of the same genus, emphasizing the difficulty in taxonomic classification of lactobacilli.
对乳酸杆菌的首次全面比较分析是通过比较植物乳杆菌（3.3兆碱基）和约氏乳杆菌（2.0兆碱基）的基因组来完成的。约氏乳杆菌主要存在于胃肠道中，而植物乳杆菌也存在于植物及植物源材料上，并用于各种工业发酵。植物乳杆菌和约氏乳杆菌的染色体只有28个基因顺序保守的区域，总计约0.75兆碱基；这些区域并非共线性，表明存在主要的染色体重排。代谢重建表明约氏乳杆菌和植物乳杆菌之间存在许多差异：约氏乳杆菌缺乏许多参与糖代谢以及氨基酸、核苷酸、脂肪酸和辅因子生物合成的酶。在假定的细胞外蛋白的数量和类型上发现了主要差异，由于它们在宿主 - 微生物相互作用中可能发挥的作用，这些差异备受关注。植物乳杆菌和约氏乳杆菌在基因组组织和基因含量方面的差异，对于同一属的两种细菌来说格外大，这凸显了乳酸杆菌分类学分类的困难。
