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Diagn Microbiol Infect Dis. 2004 Mar;48(3):201-5. doi: 10.1016/j.diagmicrobio.2003.09.008.
2
Evaluation of the Etest and disk diffusion methods for determining susceptibilities of 235 bloodstream isolates of Candida glabrata to fluconazole and voriconazole.评价Etest法和纸片扩散法测定235株光滑念珠菌血流分离株对氟康唑和伏立康唑的敏感性。
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In vitro activities of ravuconazole and voriconazole compared with those of four approved systemic antifungal agents against 6,970 clinical isolates of Candida spp.雷夫康唑和伏立康唑与四种已获批的全身性抗真菌药物对6970株念珠菌属临床分离株的体外活性比较
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5
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8
Amphotericin B resistance testing of Candida spp.: a comparison of methods.念珠菌属的两性霉素B耐药性检测：方法比较
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Comparison of Etest and National Committee for Clinical Laboratory Standards broth macrodilution method for antifungal susceptibility testing: enhanced ability to detect amphotericin B-resistant Candida isolates.用于抗真菌药敏试验的Etest法与美国国家临床实验室标准委员会肉汤稀释法的比较：增强检测两性霉素B耐药念珠菌分离株的能力。
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使用含葡萄糖和亚甲蓝的穆勒-欣顿琼脂的Etest法对4936株念珠菌属临床分离株测定两性霉素B最低抑菌浓度的评估。

Evaluation of the etest method using Mueller-Hinton agar with glucose and methylene blue for determining amphotericin B MICs for 4,936 clinical isolates of Candida species.

作者信息

Pfaller M A, Boyken L, Messer S A, Tendolkar S, Hollis R J, Diekema D J

机构信息

Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, USA.

出版信息

J Clin Microbiol. 2004 Nov;42(11):4977-9. doi: 10.1128/JCM.42.11.4977-4979.2004.

DOI:10.1128/JCM.42.11.4977-4979.2004
PMID:15528683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC525169/
Abstract

The performance of the Etest using Mueller-Hinton agar supplemented with glucose (2%) and methylene blue (0.5 microg/ml) (MH-GMB) for amphotericin B susceptibility testing of 4,936 isolates of Candida spp. was assessed against that of Etest using RPMI agar with 2% glucose (RPG). MICs were determined by Etest in both media for all 4,936 isolates and were read after incubation for 48 h at 35 degrees C. The Candida isolates included C. albicans (n = 2,728), C. glabrata (n = 722), C. parapsilosis (n = 666), C. tropicalis (n = 528), C. krusei (n = 143), C. lusitaniae (n = 54), C. guilliermondii (n = 39), C. pelliculosa (n = 17), C. kefyr (n = 15), C. rugosa (n = 11), C. dubliniensis (n = 5), C. zeylanoides (n = 4), C. lipolytica (n = 3), and C. famata (n = 1). The Etest results with MH-GMB correlated well with those with RPG. Overall agreement was 92.9%, and agreements for individual species were as follows: C. lusitaniae, 98.1%; C. albicans, 95.1%; C. glabrata, 94.3%; C. krusei, 91.6%; C. parapsilosis, 86.6%; and C. tropicalis, 86.4%. The Etest method using MH-GMB appears to be a useful method for determining amphotericin B susceptibilities of Candida species.

摘要

使用补充有葡萄糖（2%）和亚甲蓝（0.5微克/毫升）的穆勒-欣顿琼脂（MH-GMB）对4936株念珠菌属分离株进行两性霉素B药敏试验的Etest性能，与使用含2%葡萄糖的RPMI琼脂（RPG）的Etest进行了比较。对所有4936株分离株在两种培养基中均采用Etest测定最低抑菌浓度（MIC），并在35℃孵育48小时后读取结果。念珠菌分离株包括白色念珠菌（n = 2728）、光滑念珠菌（n = 722）、近平滑念珠菌（n = 666）、热带念珠菌（n = 528）、克柔念珠菌（n = 143）、葡萄牙念珠菌（n = 54）、季也蒙念珠菌（n = 39）、膜璞念珠菌（n = 17）、凯菲念珠菌（n = 15）、皱落念珠菌（n = 11）、都柏林念珠菌（n = 5）、锡兰念珠菌（n = 4）、解脂念珠菌（n = 3）和法塔念珠菌（n = 1）。MH-GMB的Etest结果与RPG的结果相关性良好。总体一致性为92.9%，各菌种的一致性如下：葡萄牙念珠菌，98.1%；白色念珠菌，95.1%；光滑念珠菌，94.3%；克柔念珠菌，91.6%；近平滑念珠菌，86.6%；热带念珠菌，86.4%。使用MH-GMB的Etest方法似乎是一种用于确定念珠菌属两性霉素B药敏性有用的方法。