Barry A L, Pfaller M A, Rennie R P, Fuchs P C, Brown S D
The Clinical Microbiology Institute, Wilsonville, Oregon 97070, USA.
Antimicrob Agents Chemother. 2002 Jun;46(6):1781-4. doi: 10.1128/AAC.46.6.1781-1784.2002.
Interpretive agreements among the results of fluconazole broth microdilution tests, Etests, and disk diffusion tests were documented by evaluating 495 Candida spp. Microdilution reference test results were in agreement with 96% of the Etest results; most discrepancies were minor differences. Fluconazole resistance of Candida krusei strains often required a full 48 h of incubation in order to be observed by the standard method. For the disk diffusion tests that were performed on Mueller-Hinton agar with glucose and methylene blue, 97% of results were in agreement with those of the reference test, especially when zones of inhibition were measured after the first 24 h of incubation. Some Candida glabrata isolates failed to grow satisfactorily until a full 48 h of incubation was completed. Precision was determined by testing 50 selected isolates in triplicate in each of three laboratories. The reproducibility of results of disk diffusion tests was comparable to that of the reference method. With all procedures, determination of test results was particularly challenging with some strains, and new methods are needed in order to improve endpoint definition.
通过评估495株念珠菌属菌株，记录了氟康唑肉汤微量稀释试验、Etest试验和纸片扩散试验结果之间的解释一致性。微量稀释参考试验结果与96%的Etest试验结果一致；大多数差异为微小差异。克鲁斯念珠菌菌株的氟康唑耐药性通常需要在标准方法下孵育整整48小时才能观察到。对于在含葡萄糖和亚甲蓝的穆勒-欣顿琼脂上进行的纸片扩散试验，97%的结果与参考试验结果一致，尤其是在孵育24小时后测量抑菌圈时。一些光滑念珠菌分离株在孵育满48小时之前生长不佳。通过在三个实验室中对50株选定的分离株进行一式三份的测试来确定精密度。纸片扩散试验结果的重现性与参考方法相当。对于所有程序，某些菌株的试验结果判定尤其具有挑战性，需要新的方法来改善终点定义。
