Overduin Pieter, Schouls Leo, Roholl Paul, van der Zanden Adri, Mahmmod Nofel, Herrewegh Arnold, van Soolingen Dick
Mycobacteria Reference Laboratory, National Institute of Public Health and the Environment, Bilthoven, The Netherlands.
J Clin Microbiol. 2004 Nov;42(11):5022-8. doi: 10.1128/JCM.42.11.5022-5028.2004.
The etiology of Crohn's disease in humans is largely unknown. Clinical signs of Crohn's disease partly resemble the clinical picture of Johne's disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis. Because of the high prevalence of these bacteria in (products of) ruminants and their remarkable thermostability, concern has been raised about the possible role of these bacteria in the pathogenesis of Crohn's disease. In an attempt to develop a molecular typing method to facilitate meaningful comparative DNA fingerprinting of M. avium subsp. paratuberculosis isolates from the human and animal reservoirs, multilocus variable-number tandem-repeat analysis (MLVA) was explored and compared to IS900 restriction fragment length polymorphism (RFLP) typing. MLVA typing subdivided the most predominant RFLP type, R01, into six subtypes and thus provides a promising molecular subtyping approach to study the diversity of M. avium subsp. paratuberculosis.
人类克罗恩病的病因在很大程度上尚不清楚。克罗恩病的临床症状部分类似于由副结核分枝杆菌引起的反刍动物副结核病的临床表现。由于这些细菌在反刍动物（及其产品）中的高流行率以及它们显著的热稳定性，人们对这些细菌在克罗恩病发病机制中可能发挥的作用表示担忧。为了开发一种分子分型方法，以便对来自人类和动物宿主的副结核分枝杆菌分离株进行有意义的比较DNA指纹分析，研究人员探索了多位点可变数目串联重复分析（MLVA），并将其与IS900限制性片段长度多态性（RFLP）分型进行比较。MLVA分型将最主要的RFLP类型R01细分为六个亚型，因此为研究副结核分枝杆菌的多样性提供了一种有前景的分子亚型分析方法。
