Jin Jinghua, Haga Takeshi, Shinjo Toshiharu, Goto Yoshitaka
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
J Vet Med Sci. 2004 Oct;66(10):1243-5. doi: 10.1292/jvms.66.1243.
The nucleotide sequences of the DNA gyrase B subunit gene (gyrB) of Fusobacterium necrophorum subsp. necrophorum, F. necrophorum subsp. funduliforme and F. varium were determined and analyzed together with those of F. nucleatum subsp. nucleatum and F. nucleatum subsp. vincentii. On the phylogenetic tree constructed, the strains of each fusobacterial species formed distinct clusters with deep sublines. The degree of sequence similarity within each cluster was 93.2% or more, whereas similarities between clusters ranged from 70.1 to 72.7%. These clusters were recovered with 100% bootstrap probabilities and are in very good agreement with the species of Fusobacterium. These data suggest that gyrB is an accurate genealogical marker for the classification of the fusobacterial taxa considered in this study.
测定并分析了坏死梭杆菌坏死亚种、坏死梭杆菌底形亚种和可变梭杆菌DNA促旋酶B亚基基因（gyrB）的核苷酸序列，并将其与具核梭杆菌具核亚种和具核梭杆菌文森特亚种的序列进行了比较。在构建的系统发育树上，每个梭杆菌属物种的菌株形成了具有深亚系的不同簇。每个簇内的序列相似性为93.2%或更高，而簇间相似性范围为70.1%至72.7%。这些簇的自展概率为100%，与梭杆菌属的物种非常吻合。这些数据表明，gyrB是本研究中所考虑的梭杆菌分类单元的准确谱系标记。
