Anwar Muhammad Saeed, Jaffery Ghazala, Rehman Bhatti Khalil-Ur-, Tayyib Muhammad, Bokhari Shahid Raza
Department of Pathology, Postgraduate Medical Institute, Lahore.
J Coll Physicians Surg Pak. 2004 Nov;14(11):661-4.
Assessment of Staphylococcus aureus (S. aureus) and MRSA nasal carriage in our general population.
Cross-sectional analytical study.
A private Pathology Laboratory in Lahore city from January 2002 to December 2003.
Nasal swabs were obtained from the apparently healthy persons accompanying the patients attending the laboratory. These swabs were inoculated on blood and mannitol salt agar plates and incubated at 37 degrees C. The isolate(s) were identified as S. aureus on the basis of colony morphology, Gram staining, catalase and coagulase tests. Sensitivity to Oxacillin was determined using standard Kirby Bauer technique.
Nasal swabs from 1660 subjects from the community were studied. Out of these samples, a total of 246 (14.82%) samples were positive for growth of S. aureus (nasal carriers of S. aureus). Out of 246 S. aureus isolates, 48 (19.51%) isolates were methicillin resistant S. aureus (MRSA). Nasal carriage was higher in males (15.47%) as compared to females (13.26%), in urban areas (16.99%) as compared to rural areas (11.32%) and in the year 2002 (16.02%) as compared to year 2003 (13.08%). However, the difference was significant only for the urban vs. rural comparison (p<0.05). MRSA isolates were also significantly higher among S. aureus isolates from the nasal cavities of urban subjects (22.98%) as compared to rural ones (11.11%). Maximum nasal carriage was present in the age group upto 9 years (20.23%) with decrease in the age groups 10-19, 20-29 and 30-39 years followed by small rise in the older subjects.
The nasal S. aureus carriage as well as methicillin resistance among these isolates are more common in urban community.
评估普通人群中金黄色葡萄球菌（S. aureus）和耐甲氧西林金黄色葡萄球菌（MRSA）的鼻腔定植情况。
横断面分析研究。
2002年1月至2003年12月在拉合尔市的一家私立病理实验室。
从到该实验室就诊患者的陪同人员中表面健康的个体获取鼻拭子。将这些拭子接种在血平板和甘露醇盐琼脂平板上，于37℃孵育。根据菌落形态、革兰氏染色、过氧化氢酶和凝固酶试验将分离株鉴定为金黄色葡萄球菌。采用标准的 Kirby Bauer 技术测定对苯唑西林的敏感性。
对来自社区的1660名受试者的鼻拭子进行了研究。在这些样本中，共有246份（14.82%）样本金黄色葡萄球菌生长呈阳性（金黄色葡萄球菌鼻腔定植者）。在246株金黄色葡萄球菌分离株中，48株（19.51%）为耐甲氧西林金黄色葡萄球菌（MRSA）。男性的鼻腔定植率（15.47%）高于女性（13.26%），城市地区（16.99%）高于农村地区（11.32%），2002年（16.02%）高于2003年（13.08%）。然而，仅城市与农村的比较差异具有统计学意义（p<0.05）。城市受试者鼻腔金黄色葡萄球菌分离株中的MRSA分离株（22.98%）也显著高于农村受试者（11.11%）。9岁及以下年龄组的鼻腔定植率最高（20.23%），10 - 19岁、20 - 29岁和30 - 39岁年龄组的定植率下降，随后老年受试者中有小幅上升。
这些分离株中的鼻腔金黄色葡萄球菌定植以及耐甲氧西林情况在城市社区更为常见。
