Rijal K R, Pahari N, Shrestha B K, Nepal A K, Paudel B, Mahato P, Skalko-Basnet N
Department of Microbiology, Pokhara University, Pokhara, Nepal.
Nepal Med Coll J. 2008 Sep;10(3):192-5.
Present study was carried out to find out the prevalence of methicillin resistant Staphylococcus aureus (MRSA) in school children of Pokhara city in western, Nepal. A total of 184 randomly selected children younger than 15 years were included in the study. Nasal swabs collected were subjected to standard bacteriological culture. S. aureus isolates were identified by mannitol fermentation, coagulase positivity and DNase positivity. Antimicrobial susceptibility test was performed on muller-hinton agar (MHA) by modified Kirby-Bauer disc diffusion method. Out of total 184 nasal swabs, S. aureus was isolated in 31.0% (n=57). Among the isolates, 35.1% (n=20) were from male children whereas 64.9% (n=37) were from female. There was no significant sex difference in colonization of S. aureus. Out of 57 isolates, 56.1% (n=32) were MRSA. MRSA isolates indicated relatively high rate of resistance to antibiotic cloxacillin (68.7%) followed by ofloxacin (40.6%), tetracycline (15.6%), erythromycin (9.4%), ciprofloxacin (6.2%) and vancomycin (3.1%).This study showed a high prevalence of MRSA carriage in school children indicating the spread of MRSA in the community.
本研究旨在查明尼泊尔西部博卡拉市学童中耐甲氧西林金黄色葡萄球菌（MRSA）的流行情况。共有184名随机选取的15岁以下儿童纳入本研究。所采集的鼻拭子进行标准细菌培养。金黄色葡萄球菌分离株通过甘露醇发酵、凝固酶阳性和DNA酶阳性进行鉴定。采用改良的 Kirby-Bauer 纸片扩散法在 Mueller-Hinton 琼脂（MHA）上进行药敏试验。在总共184份鼻拭子中，分离出金黄色葡萄球菌的占31.0%（n = 57）。在这些分离株中，35.1%（n = 20）来自男童，64.9%（n = 37）来自女童。金黄色葡萄球菌定植在性别上无显著差异。在57株分离株中，56.1%（n = 32）为MRSA。MRSA分离株对抗生素氯唑西林的耐药率相对较高（68.7%），其次是氧氟沙星（40.6%）、四环素（15.6%）、红霉素（9.4%）、环丙沙星（6.2%）和万古霉素（3.1%）。本研究表明学童中MRSA携带率很高，提示MRSA在社区中传播。
