Paściak Mariola, Holst Otto, Lindner Buko, Mierzchała Magdalena, Grzegorzewicz Anna, Mordarska Halina, Gamian Andrzej
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, PL-53-114 Wrocław, Poland.
Biochim Biophys Acta. 2004 Nov 18;1675(1-3):54-61. doi: 10.1016/j.bbagen.2004.08.004.
Structural studies on the major glycolipid isolated from Rothia mucilaginosa were carried out utilising specific chemical degradation, NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight mass spectrometry (MALDI TOF-MS). The glycolipid was found to be a dimannosylacylmonoglyceride in which the carbohydrate part was the glycerol-linked dimannoside alpha-D-Manp-(1-->3)-alpha-D-Manp-(1-->3)-sn-Gro (Man A-Man B-Gro), of which Man B was esterified at O-6 by a fatty acid residue. A second fatty acid substituted the secondary methylene position of the glycerol residue, in contrast to the glycolipid previously found in R. dentocariosa and Saccharopolyspora strains, in which the second fatty acid esterified the primary methylene position of glycerol. Results of the ELISA experiment with rabbit specific antibacterial sera indicate that these two major glycolipids are antigenic, and the patterns of serological reactivity are similar but not identical.
利用特定化学降解、核磁共振光谱法以及基质辅助激光解吸/电离飞行时间质谱法（MALDI TOF-MS），对从粘液罗氏菌中分离出的主要糖脂进行了结构研究。该糖脂被发现是一种二甘露糖基酰基单甘油酯，其中碳水化合物部分是甘油连接的二甘露糖苷α-D-甘露糖-(1→3)-α-D-甘露糖-(1→3)-sn-甘油（Man A-Man B-甘油），其中Man B在O-6位被一个脂肪酸残基酯化。与先前在龋齿罗氏菌和糖多孢菌菌株中发现的糖脂不同，第二种脂肪酸取代了甘油残基的仲亚甲基位置，在后者中第二种脂肪酸酯化了甘油的伯亚甲基位置。用兔特异性抗菌血清进行的ELISA实验结果表明，这两种主要糖脂具有抗原性，并且血清反应模式相似但不完全相同。
