Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, Wrocław, Poland.
Carbohydr Res. 2010 Jul 19;345(11):1570-4. doi: 10.1016/j.carres.2010.04.026. Epub 2010 May 24.
The chemical structure of the major glycolipid present in the chloroform-methanol extract of bacterial biomass of Tsukamurella pulmonis is reported. This compound was purified by TLC and HPLC. The sugar analysis revealed only glucose. Detailed chemical analyses, NMR, and MALDI FT-ICR-mass spectrometric studies identified 2,3-di-O-acyl-alpha-d-glucopyranosyl-(1-->1)-alpha-d-glucopyranose as the final structure. Short branched fatty acids (4:0 or 5:0) were linked to C-3 and saturated, mono, and diunsaturated 18:0, 18:1, 18:2, 20:1, 20:2, and 20:0 to C-2 of the same glucose residue. ELISA tests revealed the weak cross-reactivity of the glycolipid with rabbit antisera against cells of T. paurometabola, Rhodococcus wratislaviensis, and Nocardiopsis dassonvillei.
本文报道了从火鸡包柔螺旋体细菌生物量的氯仿-甲醇提取物中分离得到的主要糖脂的化学结构。该化合物通过 TLC 和 HPLC 进行了纯化。糖分析仅显示葡萄糖。详细的化学分析、NMR 和 MALDI FT-ICR 质谱研究确定 2,3-二酰基-α-d-吡喃葡萄糖基-(1-->1)-α-d-吡喃葡萄糖为最终结构。短支链脂肪酸（4:0 或 5:0）连接到 C-3，而饱和、单不饱和和二不饱和的 18:0、18:1、18:2、20:1、20:2 和 20:0 连接到同一葡萄糖残基的 C-2。ELISA 试验表明，该糖脂与兔抗火鸡包柔螺旋体、威斯康星棒杆菌和游动放线菌细胞的抗血清具有较弱的交叉反应性。
