来自印度物种的产白藜芦醇可培养内生真菌的多样性与系统发育
Diversity and phylogeny of resveratrol-producing culturable endophytic fungi from species in India.
作者信息
Dwibedi Vagish, Saxena Sanjai
机构信息
Department of Biotechnology, Thapar Institute of Engineering and Technology, Thapar University, Patiala, Punjab 147001 India.
出版信息
3 Biotech. 2019 May;9(5):182. doi: 10.1007/s13205-019-1712-x. Epub 2019 Apr 22.
Abstract
In the present study, resveratrol producing culturable endophytes were recovered from the Indian vineyards. Of the 145 endophytic fungi recovered, only 30.3% were positive for resveratrol production in the preliminary screening. Culture broth of only 13 isolates exhibited the actual presence of resveratrol in the range of 4.4-52.3 μg/ml by HPLC quantification. The most potent endophytic isolate producing the highest extracellular resveratrol was #19VVLPM isolated from the Pinot Noir variety. Further LC-MS analysis confirmed the similarity of fungal resveratrol with standard resveratrol. The Merlot variety exhibited the highest colonization frequency of the resveratrol-producing fungal endophytes. Using morphological methods as well as molecular phylogeny #19VVLPM was identified as . It is a promising candidate for further development into a commercial strain.
摘要
在本研究中，从印度葡萄园分离出了能够产生白藜芦醇的可培养内生菌。在分离出的145种内生真菌中，初步筛选时只有30.3%对白藜芦醇的产生呈阳性。通过高效液相色谱定量分析，只有13株分离物的培养液中实际存在白藜芦醇，其含量在4.4 - 52.3μg/ml范围内。产生胞外白藜芦醇能力最强的内生分离株是从黑皮诺品种中分离出的#19VVLPM。进一步的液相色谱 - 质谱分析证实了真菌白藜芦醇与标准白藜芦醇的相似性。梅洛品种的产生白藜芦醇的真菌内生菌定殖频率最高。利用形态学方法以及分子系统发育分析，#19VVLPM被鉴定为 。它是进一步开发成商业菌株的有前途的候选菌株。 （注：原文中“#19VVLPM被鉴定为 ”后面缺少具体内容）
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