Clinics of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Hospital Giessen and Marburg, Justus-Liebig-University Giessen, Marburg, Federal Republic of Germany.
Amino Acids. 2011 Apr;40(4):1077-90. doi: 10.1007/s00726-010-0731-z. Epub 2010 Sep 14.
For the first time the immunonutritional role of pyruvate on neutrophils (PMN), free α-keto and amino acid profiles, important reactive oxygen species (ROS) produced [superoxide anion (O(2) (-)), hydrogen peroxide (H(2)O(2))] as well as released myeloperoxidase (MPO) acitivity has been investigated. Exogenous pyruvate significantly increased PMN pyruvate, α-ketoglutarate, asparagine, glutamine, aspartate, glutamate, arginine, citrulline, alanine, glycine and serine in a dose as well as duration of exposure dependent manner. Moreover, increases in O(2) (-) formation, H(2)O(2)-generation and MPO acitivity in parallel with intracellular pyruvate changes have also been detected. Regarding the interesting findings presented here we believe, that pyruvate fulfils considerably the criteria for a potent immunonutritional molecule in the regulation of the PMN dynamic α-keto and amino acid pools. Moreover it also plays an important role in parallel modulation of the granulocyte-dependent innate immune regulation. Although further research is necessary to clarify pyruvate's sole therapeutical role in critically ill patients' immunonutrition, the first scientific successes seem to be very promising.
首次研究了丙酮酸对中性粒细胞（PMN）、游离α-酮酸和氨基酸谱的免疫营养作用，以及重要的活性氧物质（ROS）的产生[超氧阴离子（O(2) (-)）、过氧化氢（H(2)O(2)）]和髓过氧化物酶（MPO）活性的释放。外源性丙酮酸显著增加了 PMN 中的丙酮酸、α-酮戊二酸、天冬酰胺、谷氨酰胺、天冬氨酸、谷氨酸、精氨酸、瓜氨酸、丙氨酸、甘氨酸和丝氨酸，其增加程度与暴露时间和剂量有关。此外，还检测到与细胞内丙酮酸变化平行的 O(2) (-) 形成、H(2)O(2)-生成和 MPO 活性的增加。鉴于这里提出的有趣发现，我们认为丙酮酸在调节 PMN 动态α-酮酸和氨基酸池方面符合有效的免疫营养分子标准。此外，它在粒细胞依赖性先天免疫调节的平行调节中也起着重要作用。尽管有必要进行进一步的研究来阐明丙酮酸在危重病患者免疫营养中的单一治疗作用，但最初的科学成功似乎非常有前途。
