Kumral Abdullah, Genc Sermin, Genc Kursad, Duman Nuray, Tatli Mansur, Sakizli Meral, Ozkan Hasan
Department of Pediatrics, Dokuz Eylul University, Inciralti, TR-35340 Izmir, Turkey.
Biol Neonate. 2005;87(2):99-104. doi: 10.1159/000081969. Epub 2004 Nov 9.
High levels of unconjugated bilirubin can be neurotoxic and gliotoxic. However, the effect of serum from patients with neonatal indirect hyperbilirubinemia on astrocyte viability has never been investigated.
In the present study, we searched for the possible toxic effect of hyperbilirubinemic serum on murine astrocytes.
Heat-inactivated patient serum was added to astrocyte cultures at different concentrations varying from 1 to 20%, and cultures were incubated for 24, 48, and 72 h. Sera from healthy infants without hyperbilirubinemia were used as controls. Cytotoxicity was evaluated according to the release of lactate dehydrogenase in the culture medium. Apoptotic cell death was determined by anti-single-strand DNA immunostaining.
The results of the present study show that hyperbilirubinemic serum induces cytotoxicity and apoptotic astrocyte death in a concentration- and time-dependent manner.
We conclude that serum from patients with neonatal indirect hyperbilirubinemia is cytotoxic to murine astrocytes.
高水平的未结合胆红素具有神经毒性和神经胶质毒性。然而，新生儿间接高胆红素血症患者的血清对星形胶质细胞活力的影响从未被研究过。
在本研究中，我们探寻高胆红素血症血清对小鼠星形胶质细胞可能的毒性作用。
将热灭活的患者血清以1%至20%的不同浓度添加到星形胶质细胞培养物中，并将培养物孵育24、48和72小时。来自无高胆红素血症的健康婴儿的血清用作对照。根据培养基中乳酸脱氢酶的释放来评估细胞毒性。通过抗单链DNA免疫染色确定凋亡细胞死亡。
本研究结果表明，高胆红素血症血清以浓度和时间依赖性方式诱导细胞毒性和星形胶质细胞凋亡死亡。
我们得出结论，新生儿间接高胆红素血症患者的血清对小鼠星形胶质细胞具有细胞毒性。
