Chuniaud L, Dessante M, Chantoux F, Blondeau J P, Francon J, Trivin F
Service de Biochimie, Fondation Hôpital Saint Joseph, Paris, France.
Clin Chim Acta. 1996 Dec 30;256(2):103-14. doi: 10.1016/s0009-8981(96)06407-8.
The sensitivity of rat brain astrocytes and human fibroblasts in culture to unconjugated bilirubin was investigated. Medium containing 6 mumol/1 bilirubin and increasing concentrations of human serum albumin giving ratios of 0.5-1.5 that resulted in an increase of the free bilirubin concentrations. The LDH activity in the culture medium was an index of cytolysis and the MTT assay was used as an index of mitochondrial impairment. The ratios producing half-maximum cell lysis after 24, 48, and 72 h, were 1.1, 0.9 and 0.85, for astrocytes, and 1.2, 0.75 and 0.75, for fibroblasts. Mitochondrial activity decreased after 24 h for ratio = 0.7 and partly recovered at 48 h. Mitochondrial activity was more impaired in fibroblasts than in astrocytes above ratio = 0.7. The cytotoxic effects were linked to the free bilirubin concentration. We conclude that astrocytes are less sensitive to bilirubin cytotoxic effects than are fibroblasts.
研究了培养的大鼠脑星形胶质细胞和人成纤维细胞对未结合胆红素的敏感性。培养基中含有6 μmol/L胆红素，并增加人血清白蛋白的浓度，使比例为0.5 - 1.5，从而导致游离胆红素浓度增加。培养基中的乳酸脱氢酶（LDH）活性是细胞溶解的指标，MTT法用作线粒体损伤的指标。在24、48和72小时后产生半数最大细胞溶解的比例，星形胶质细胞分别为1.1、0.9和0.85，成纤维细胞分别为1.2、0.75和0.75。当比例 = 0.7时，24小时后线粒体活性下降，并在48小时部分恢复。当比例高于0.7时，成纤维细胞中的线粒体活性比星形胶质细胞受到的损伤更大。细胞毒性作用与游离胆红素浓度有关。我们得出结论，星形胶质细胞对胆红素细胞毒性作用的敏感性低于成纤维细胞。
