Schleiss Mark R, Bernstein David I, Passo Murray, Parker Susan, Meric Claude, Verdier François, Newkirk Marianna M
Division of Infectious Diseases, 3333 Burnet Avenue, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Vaccine. 2004 Dec 16;23(5):687-92. doi: 10.1016/j.vaccine.2004.06.038.
Possible correlations have been proposed between autoimmune diseases, such as systemic lupus erythematosus (SLE), and infection with human cytomegalovirus (CMV). The recent observation that an adenovirus expressing the immunodominant envelope glycoprotein of CMV, glycoprotein B (gB), may be capable of inducing autoantibodies in certain mouse strains has prompted interest in exploring potential relationships between gB immunization and autoimmune disease. We examined whether a recombinant CMV gB vaccine, or a gB canarypox vectored vaccine (ALVAC-CMVgB), administered to a total of 76 CMV-seronegative subjects, was capable of inducing cross-reactive antibodies to Smith antigen (Sm), ribonucleoprotein complex (RNP), and the U1-70 kDa component of the RNP complex. Using immunofluorescence, EIA and immunoblot analyses, we failed to identify induction of autoantibodies following vaccination with gB, whether administered alone as a purified protein subunit with adjuvant, or in combination with expression in a vectored approach using a recombinant canarypox. These data reinforce the favorable safety profile of CMV gB vaccines.
自身免疫性疾病，如系统性红斑狼疮（SLE），与人巨细胞病毒（CMV）感染之间可能存在相关性。最近观察到，一种表达CMV免疫显性包膜糖蛋白糖蛋白B（gB）的腺病毒可能能够在某些小鼠品系中诱导自身抗体，这引发了人们对探索gB免疫与自身免疫性疾病之间潜在关系的兴趣。我们研究了给予总共76名CMV血清阴性受试者的重组CMV gB疫苗或gB金丝雀痘病毒载体疫苗（ALVAC-CMVgB）是否能够诱导针对史密斯抗原（Sm）、核糖核蛋白复合物（RNP）以及RNP复合物的U1-70 kDa成分的交叉反应性抗体。通过免疫荧光、酶免疫分析和免疫印迹分析，我们未能确定接种gB后是否会诱导自身抗体，无论gB是作为纯化的蛋白亚基与佐剂单独给药，还是与重组金丝雀痘病毒载体表达联合给药。这些数据强化了CMV gB疫苗良好的安全性。
