Ignatova Milena, Voccia Samuel, Gilbert Bernard, Markova Nadya, Mercuri Paola Sandra, Galleni Moreno, Sciannamea Valérie, Lenoir Sandrine, Cossement Damien, Gouttebaron Rachel, Jérôme Robert, Jérôme Christine
Center for Education and Research on Macromolecules (CERM), University of Liège, Sart-Tilman, B6, B-4000 Liège, Belgium.
Langmuir. 2004 Nov 23;20(24):10718-26. doi: 10.1021/la048347t.
Novel copolymer brushes have been synthesized by a two-step "grafting from" method that consists of the electrografting of poly(2-phenyl-2-(2,2,6,6-tetramethyl-piperidin-1-yloxy)-ethylacrylate) onto stainless steel, followed by the nitroxide-mediated radical polymerization of 2-(dimethylamino ethyl)acrylate and styrene or n-butyl acrylate, initiated from the electrografted polyacrylate chains. The grafted copolymers were quaternized in order to endow them with antibacterial properties. Peeling tests have confirmed the strong adhesion of the grafted copolymer onto the stainless steel substrate. Quartz crystal microbalance experiments have proven that fibrinogen adhesion is lower on the hydrophilic quaternized films compared to the nonionic counterpart. Such quaternized copolymers exhibit significant antibacterial activity against the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli.
通过两步“接枝自”法合成了新型共聚物刷，该方法包括将聚（2-苯基-2-（2,2,6,6-四甲基哌啶-1-氧基）-乙基丙烯酸酯）电接枝到不锈钢上，然后从电接枝的聚丙烯酸酯链引发2-（二甲基氨基乙基）丙烯酸酯和苯乙烯或丙烯酸正丁酯的氮氧自由基介导的自由基聚合。对接枝共聚物进行季铵化以赋予其抗菌性能。剥离试验证实了接枝共聚物在不锈钢基底上的强附着力。石英晶体微天平实验证明，与非离子对应物相比，亲水性季铵化膜上的纤维蛋白原附着力更低。这种季铵化共聚物对革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性菌大肠杆菌具有显著的抗菌活性。
