College of Chemical Engineering, Sichuan University , Chengdu 610065, China.
Langmuir. 2011 Mar 15;27(6):2761-74. doi: 10.1021/la104442f. Epub 2011 Feb 21.
An environmentally benign approach to impart stainless steel (SS) surfaces with antifouling and antibacterial functionalities was described. Surface-initiated atom transfer radical polymerization (ATRP) of poly(ethylene glycol) monomethacrylate) (PEGMA) from the SS surface-coupled catecholic L-3,4-dihydroxyphenylalanine (DOPA) with terminal alkyl halide initiator was first carried out, followed by the immobilization of lysozyme at the chain ends of poly(ethylene glycol) branches of the grafted PEGMA polymer brushes. The functionalized SS surfaces were shown to be effective in preventing bovine serum albumin (BSA) adsorption and in reducing bacterial adhesion and biofilm formation. The surfaces also exhibited good bactericidal effects against Escherichia coli and Staphylococcus aureus. The concomitant incorporation of antifouling hydrophilic brushes and antibacterial enzymes or peptides onto metal surfaces via catecholic anchors should be readily adaptable to other metal substrates, and is potentially useful for biomedical and biomaterial applications.
本文描述了一种将不锈钢（SS）表面赋予抗污和抗菌功能的环保方法。首先，通过 SS 表面偶联儿茶酚 L-3,4-二羟基苯丙氨酸（DOPA）与末端烷基卤引发剂，从 SS 表面引发聚乙二醇单甲醚（PEGMA）的原子转移自由基聚合（ATRP），然后将溶菌酶固定在接枝 PEGMA 聚合物刷的聚乙二醇支链的链末端。功能化的 SS 表面在防止牛血清白蛋白（BSA）吸附和减少细菌黏附和生物膜形成方面表现出有效。该表面对大肠杆菌和金黄色葡萄球菌也具有良好的杀菌作用。通过儿茶酚配体将抗污亲水刷和抗菌酶或肽共同整合到金属表面上，应该很容易适用于其他金属基底，并且在生物医学和生物材料应用中具有潜在的用途。
