解淀粉芽胞杆菌（Paenibacillus oleatilyticus），从土壤中分离得到。
Paenibacillus oleatilyticus sp. nov., isolated from soil.
机构信息
Department of Biochemistry, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus road, Pashan, Pune, 411027, Maharashtra, India.
出版信息
Arch Microbiol. 2022 Jul 22;204(8):516. doi: 10.1007/s00203-022-03116-0.
A gram-stain-negative, endo-spore forming, facultatively anaerobic, motile, rod-shaped bacterial strain SM69, isolated from soil samples of Rohtak, Haryana, India was characterized using polyphasic approach. White colonies were 2-3 mm, in diameter and growth occurred between 20 and 55 °C, pH 6.0-10.0 with 0-2.0% (w/v) NaCl. Based on 16S rRNA gene sequence similarity the strain is placed in the genus Paenibacillus as it is closely related to 'Paenibacillus tyrfis MSt1' (99.7%) and P. elgii SD17 (99.6%). The cell wall peptidoglycan contained meso-diaminopimelic acid. The dominant fatty acids included anteiso-C (50%), C (12%) and anteiso-C (10%). Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The size of the draft genome was 7,848,017 bp, with 53.1% G+C content. dDDH (51.6%) and ANI (93.5%) of strain SM69 with its close relatives indicates that it represents a novel species, for which the name Paenibacillus oleatilyticus sp. nov. (Type strain SM69 = MCC 3064 = JCM 33981 = KACC 21649) is proposed.
从印度哈里亚纳邦罗塔克的土壤样本中分离到一株革兰氏阴性、内生孢子形成、兼性厌氧、能动、杆状细菌菌株 SM69，采用多相分类法对其进行了表征。白色菌落直径为 2-3mm，生长温度为 20-55°C，pH 值为 6.0-10.0，盐度为 0-2.0%（w/v）。根据 16S rRNA 基因序列相似性，该菌株属于类芽孢杆菌属，与“Paenibacillus tyrfis MSt1”（99.7%）和 P. elgii SD17（99.6%）最为密切相关。细胞壁肽聚糖含有meso-二氨基庚二酸。主要脂肪酸包括 anteiso-C（50%）、C（12%）和 anteiso-C（10%）。主要极性脂类为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。该菌株的基因组草图大小为 7848017bp，GC 含量为 53.1%。与近缘菌株的 dDDH（51.6%）和 ANI（93.5%）表明，它代表了一个新种，因此命名为解油类芽孢杆菌 Paenibacillus oleatilyticus sp. nov.（模式菌株 SM69= MCC 3064=JCM 33981=KACC 21649）。
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