Ivanova Elena P, Christen Richard, Alexeeva Yulia V, Zhukova Natalia V, Gorshkova Natalia M, Lysenko Anatoly M, Mikhailov Valery V, Nicolau Dan V
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, Russia.
Industrial Research Institute, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2107-2111. doi: 10.1099/ijs.0.02867-0.
Two whitish yellow, Gram-positive, non-motile, aerobic bacteria were isolated from enrichment culture during degradation of the thallus of the brown alga Fucus evanescens. The bacteria studied were chemo-organotrophic, mesophilic and grew well on nutrient media containing up to 15 % (w/v) NaCl. The DNA G+C content was 61 mol%. The two isolates exhibited a conspecific DNA-DNA relatedness value of 98 %, indicating that they belong to the same species. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 3637(T) formed a distinct phyletic lineage in the genus Brevibacterium (family Brevibacteriaceae, class Actinobacteria) and showed the highest sequence similarity (about 97 %) to Brevibacterium casei. DNA-DNA hybridization experiments demonstrated 45 % binding with the DNA of B. casei DSM 20657(T). Physiological and chemotaxonomic characteristics (meso-diaminopimelic acid in the peptidoglycan, major cellular fatty acids 15 : 0ai and 17 : 0ai) of the bacteria studied were consistent with the genomic and phylogenetic data. On the basis of the results of this study, a novel species, Brevibacterium celere sp. nov., is proposed. The type strain is KMM 3637(T) (=DSM 15453(T)=ATCC BAA-809(T)).
从褐藻消失墨角藻叶状体降解过程的富集培养物中分离出两株淡黄白色、革兰氏阳性、无运动性、需氧的细菌。所研究的细菌为化能有机营养型、嗜温菌，在含高达15%（w/v）氯化钠的营养培养基上生长良好。DNA G+C含量为61摩尔%。这两株分离物的种内DNA-DNA相关性值为98%，表明它们属于同一物种。16S rRNA基因序列的比较分析表明，菌株KMM 3637(T)在短杆菌属（短杆菌科，放线菌纲）中形成了一个独特的系统发育谱系，与干酪短杆菌的序列相似性最高（约97%）。DNA-DNA杂交实验表明与干酪短杆菌DSM 20657(T)的DNA有45%的结合。所研究细菌的生理和化学分类特征（肽聚糖中的内消旋二氨基庚二酸，主要细胞脂肪酸15:0ai和17:0ai）与基因组和系统发育数据一致。基于本研究结果，提出了一个新物种——敏捷短杆菌（Brevibacterium celere sp. nov.）。模式菌株为KMM 3637(T)（=DSM 15453(T)=ATCC BAA-809(T)）。
