Castro-Fernández C, Maya-Núñez G, Méndez J P
Research Unit in Developmental Biology, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México, D.F. 06703, Mexico.
Endocrine. 2004 Oct;25(1):49-54. doi: 10.1385/endo:25:1:49.
Follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) belong to the super-family of G protein-coupled receptors (GPCR); GPCRs are negatively regulated by RGS ("regulators of G protein signaling") proteins. In this study we evaluated the effects of RGS3 and RGS10 on FSHR and LHR ligand binding and effector coupling. FSHR and LHR ligand binding were unchanged in the presence of RGS3 or RGS10. However, signaling by FSHR and LHR was diminished by RGS3 but not by RGS10. This constitutes the first demonstration of an interaction between RGS proteins and LH and FSH signaling pathways and identifies a mechanism for negative regulation of RGS3 on FSHR and LHR signaling.
促卵泡激素受体（FSHR）和促黄体生成素受体（LHR）属于G蛋白偶联受体（GPCR）超家族；GPCR受RGS（“G蛋白信号调节剂”）蛋白的负调控。在本研究中，我们评估了RGS3和RGS10对FSHR和LHR配体结合及效应器偶联的影响。在RGS3或RGS10存在的情况下，FSHR和LHR的配体结合未发生变化。然而，FSHR和LHR的信号传导被RGS3减弱，但未被RGS10减弱。这首次证明了RGS蛋白与LH和FSH信号通路之间的相互作用，并确定了RGS3对FSHR和LHR信号进行负调控的机制。
