Hsu Dur-Zong, Liu Ming-Yie
Department of Environmental and Occupational Health and Institute of Basic Medical Sciences, National Cheng Kung University Medical College, Tainan, Taiwan.
Shock. 2004 Dec;22(6):582-5. doi: 10.1097/01.shk.0000135254.21699.45.
The aim of this study was to investigate effects of sesame oil on oxidative stress after the onset of sepsis in rats. Effects of sesame oil on lipid peroxidation, superoxide anion, superoxide dismutase, catalase, glutathione, and nitrite after the onset of endotoxin intoxication were determined. To further examine the protective effect of sesame oil on sepsis, a mortality study was also conduced in cecal ligation and puncture-induced sepsis in rats. Sesame oil was given orally 6 h after endotoxin administration and cecal ligation and puncture, and parameters were then measured in another 6 h. Data demonstrated that a single dose of sesame oil reduced lipid peroxidation 6 h after endotoxin intoxication. Superoxide anion counts were decreased, glutathione levels were increased, and activities of superoxide dismutase and catalase, as well as nitrite levels, were not altered in lipopolysaccharide plus sesame oil-treated groups compared with lipopolysaccharide-treated groups. Furthermore, sesame oil given 6 h after cecal ligation and puncture significantly increased survival rate. Thus, we suggested that sesame oil could be used as a potent antioxidant to reduce oxidative stress after the onset of sepsis in rats.
本研究旨在探讨芝麻油对大鼠脓毒症发作后氧化应激的影响。测定了内毒素中毒发作后芝麻油对脂质过氧化、超氧阴离子、超氧化物歧化酶、过氧化氢酶、谷胱甘肽和亚硝酸盐的影响。为进一步研究芝麻油对脓毒症的保护作用，还对盲肠结扎和穿刺诱导的大鼠脓毒症进行了死亡率研究。在内毒素给药以及盲肠结扎和穿刺后6小时口服芝麻油，然后在另外6小时测量各项参数。数据表明，单剂量芝麻油可降低内毒素中毒6小时后的脂质过氧化。与脂多糖治疗组相比，脂多糖加芝麻油治疗组的超氧阴离子计数减少，谷胱甘肽水平升高，超氧化物歧化酶和过氧化氢酶的活性以及亚硝酸盐水平未改变。此外，盲肠结扎和穿刺后6小时给予芝麻油可显著提高存活率。因此，我们认为芝麻油可作为一种有效的抗氧化剂，以减轻大鼠脓毒症发作后的氧化应激。
