Perez-Garcia C G, Tissir F, Goffinet A M, Meyer G
Department of Anatomy, University La Laguna, 38071 La Laguna, Tenerife, Spain.
Eur J Neurosci. 2004 Nov;20(10):2827-32. doi: 10.1111/j.1460-9568.2004.03733.x.
Reelin is an extracellular matrix protein secreted by a variety of cell types throughout the developing brain. The target cells for reelin express the cytoplasmic adapter protein Dab1, which binds to the reelin receptors VLDLR and ApoER2. In the present work, we have studied the localization of both receptors in developing mouse and human cortex, olfactory bulb and cerebellum. In mouse, some Cajal-Retzius cells express reelin and VLDLR; in humans, all the components of the signalling pathway (Reelin, Dab1, VLDLR and ApoER2) are present in subsets of Cajal-Retzius cells. In the mouse cortical plate, VLDLR and ApoER2 are present from E15 to postnatal stages; in human cortical plate they are most prominent at approximately 20 gestational weeks. In mice, cerebellar Purkinje cells only express VLDLR whereas in humans they express both VLDLR and ApoER2. Mitral cells of the mouse olfactory bulb are ApoER2-positive and VLDLR-negative. In sum, the receptor expression patterns are similar in the human and mouse cortical plate but differ in Cajal-Retzius and Purkinje cells, which in humans express additional components of the reelin-Dab1 pathway.
Reelin是一种细胞外基质蛋白，由发育中的大脑中的多种细胞类型分泌。Reelin的靶细胞表达细胞质衔接蛋白Dab1，该蛋白与Reelin受体VLDLR和ApoER2结合。在本研究中，我们研究了这两种受体在发育中的小鼠和人类皮质、嗅球和小脑中的定位。在小鼠中，一些Cajal-Retzius细胞表达Reelin和VLDLR；在人类中，信号通路的所有成分（Reelin、Dab1、VLDLR和ApoER2）都存在于Cajal-Retzius细胞亚群中。在小鼠皮质板中，VLDLR和ApoER2从胚胎第15天到出生后阶段都存在；在人类皮质板中，它们在大约妊娠20周时最为突出。在小鼠中，小脑浦肯野细胞仅表达VLDLR，而在人类中它们同时表达VLDLR和ApoER2。小鼠嗅球的二尖瓣细胞ApoER2呈阳性而VLDLR呈阴性。总之，人类和小鼠皮质板中的受体表达模式相似，但在Cajal-Retzius细胞和浦肯野细胞中有所不同，在人类中这些细胞表达Reelin-Dab1通路的其他成分。
