Saleem Rubeena, Ahmad Mohammad, Naz Aneela, Siddiqui Humaira, Ahmad Syed Iqbal, Faizi Shaheen
Dr. HMI Institute of Pharmacology and Herbal Sciences, Hamdard University, Karachi-74600, Pakistan.
Arch Pharm Res. 2004 Oct;27(10):1037-42. doi: 10.1007/BF02975428.
Study of the effects of the methanolic extract of Tagetes patula roots on blood pressure led to the isolation of well known citric (1) and malic acid (7) as hypotensive, and pyridine hydrochloride (4) as hypertensive constituents of the plant along with a new constituent, 2-hydroxy, 5-hydroxymethyl furan (9). Citric acid and malic acid caused 71% and 43% fall in Mean Arterial Blood Pressure (MABP) of rats at the doses of 15 mg/kg and 30 mg/kg respectively while pyridine hydrochloride produced 34% rise in the MABP of rats at the dose of 30 mg/kg. LD50 and LD100 of citric acid in mice have been determined as 545 mg/kg and 1000 mg/kg, respectively.
对孔雀草根部甲醇提取物对血压影响的研究导致分离出了已知的柠檬酸（1）和苹果酸（7）作为降压成分，以及盐酸吡啶（4）作为该植物的升压成分，同时还分离出一种新成分2-羟基-5-羟甲基呋喃（9）。柠檬酸和苹果酸分别以15mg/kg和30mg/kg的剂量使大鼠平均动脉血压（MABP）下降了71%和43%，而盐酸吡啶在30mg/kg的剂量下使大鼠的MABP升高了34%。已确定柠檬酸在小鼠中的半数致死量（LD50）和绝对致死量（LD100）分别为545mg/kg和1000mg/kg。
