College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
Department of Pharmacy, Shaheed Benazir Bhutto Sheringal Dir Upper, Khyber PakhtoonKhwa 18000, Pakistan.
Molecules. 2019 Oct 30;24(21):3911. doi: 10.3390/molecules24213911.
L. is a widely cultivated herbal medicinal plant in China and other countries. In this study, two new 2, 3-dihydrobenzofuran glucosides (, ) and fourteen known metabolites (-) were isolated from the stems and leaves of (SLT). The chemical structures of the isolated compounds were characterized comprehensively based on one- and two-dimensional NMR spectroscopy and high resolution mass spectrometry. Absolute configurations of compounds and were determined by ECD calculations. Compounds and exhibited moderate in vitro inhibitory activities against human gastric cancer cell lines (AGS) with IC values of 41.20 μmol/L and 30.43 μmol/L, respectively. The fingerprint profiles of stems and leaves of with three color types of flowers (Janie Yellow Bright, Jinmen Orange, Shouyao Red and Yellow color) were established by high-performance liquid chromatography (HPLC). Ten different batches of stems and leaves were examined as follow: Shouyao Red and Yellow color (1, 2, 3), Janie Yellow Bright (4, 5, 6, 7) and Jinmen Orange (8, 9, 10). Twenty-two common peaks were identified with similarity values ranging from 0.910 to 0.977. Meanwhile, the average peak area of SLT in the three types of flowers was different and it was the highest in Janie Yellow Bright.
L. 是一种在中国和其他国家广泛种植的草药植物。在本研究中,从 (SLT)的茎和叶中分离得到了两种新的 2,3-二氢苯并呋喃葡萄糖苷(、)和十四个已知代谢物(-)。通过一维和二维 NMR 光谱和高分辨率质谱对分离得到的化合物的化学结构进行了全面表征。通过 ECD 计算确定了化合物和的绝对构型。化合物和对人胃癌细胞系(AGS)表现出中等体外抑制活性,IC 值分别为 41.20 μmol/L 和 30.43 μmol/L。通过高效液相色谱(HPLC)建立了三种花色(Janie Yellow Bright、Jinmen Orange、Shouyao Red 和 Yellow color)的茎和叶的指纹图谱。检查了十个不同批次的茎和叶:Shouyao Red 和 Yellow color(1、2、3)、Janie Yellow Bright(4、5、6、7)和 Jinmen Orange(8、9、10)。鉴定出 22 个共有峰,相似度值在 0.910 到 0.977 之间。同时,三种花色中 SLT 的平均峰面积不同,在 Janie Yellow Bright 中最高。