内生萘并吡喃酮代谢产物是黄嘌呤氧化酶、SW1116细胞和一些微生物生长的协同抑制剂。

Endophytic naphthopyrone metabolites are co-inhibitors of xanthine oxidase, SW1116 cell and some microbial growths.

作者信息

Song Y C, Li H, Ye Y H, Shan C Y, Yang Y M, Tan R X

机构信息

Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.

出版信息

FEMS Microbiol Lett. 2004 Dec 1;241(1):67-72. doi: 10.1016/j.femsle.2004.10.005.

DOI:10.1016/j.femsle.2004.10.005

PMID:15556711

Abstract

Fractionation of the extract of Aspergillus niger. IFB-E003, an endophyte in Cyndon dactylon, gave four known compounds naphtho-gamma-pyrones rubrofusarin B, fonsecinone A, asperpyrone B and aurasperone A, which were further investigated biologically. Rubrofusarin B was shown to be cytotoxic to the colon cancer cell line SW1116 (IC50: 4.5 microgml-1), and aurasperone A inhibitory on XO (xanthine oxidase) (IC50: 10.9 micromoll-1). Moreover, the four naphtho-gamma-pyrones exhibited growth inhibitions against the five test microbes with MICs ranging in between 1.9 and 31.2 microgml(-1). The present recognition of rubrofusarin B and aurasperone A as strong co-inhibitors on XO, colon cancer cell and some microbial pathogens is of significance for the imperative discovery of new relevant therapeutic agents.

摘要

对黑麦草内生真菌黑曲霉IFB-E003提取物进行分离，得到了四种已知化合物萘并γ-吡喃酮类的红镰孢菌素B、丰塞酮A、曲霉吡喃酮B和金精曲霉酮A，并对其进行了进一步的生物学研究。结果表明，红镰孢菌素B对结肠癌细胞系SW1116具有细胞毒性（IC50：4.5 μg/ml-1），金精曲霉酮A对黄嘌呤氧化酶（XO）具有抑制作用（IC50：10.9 μmol/l-1）。此外，这四种萘并γ-吡喃酮对五种受试微生物均有生长抑制作用，MIC值在1.9至31.2 μg/ml-1之间。目前将红镰孢菌素B和金精曲霉酮A认定为XO、结肠癌细胞和一些微生物病原体的强效协同抑制剂，对于迫切需要发现新的相关治疗药物具有重要意义。