Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), Hanoi 10072, Vietnam.
Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 10072, Vietnam.
G3 (Bethesda). 2023 Feb 9;13(2). doi: 10.1093/g3journal/jkac300.
Penicillium oxalicum has been reported as a multienzyme-producing fungus and is widely used in industry due to great potential for cellulase release. Until now, there are only 10 available genome assemblies of P. oxalicum species deposited in the GenBank database. In this study, the genome of the I1R1 strain isolated from the root of Ixora chinensis was completely sequenced by Pacbio Sequel sequencing technology, assembled into 8 chromosomes with the genome size of 30.8 Mb, as well as a mitogenome of 26 kb. The structural and functional analyses of the I1R1 genome revealed gene model annotations encoding an enzyme set involved in significant metabolic processes, along with cytochrome P450s and secondary metabolite biosynthesis. The comparative analysis of the P. oxalicum species based on orthology and gene family duplications indicated their large and closed pan-genome of 9,500 orthologous groups. This is valuable data for future phylogenetic and population genomics studies.
草酸青霉已被报道为一种多酶产生真菌，由于其具有巨大的纤维素酶释放潜力，因此被广泛应用于工业领域。迄今为止，GenBank 数据库中仅存储了 10 个可用的草酸青霉种的基因组组装。在这项研究中，从龙船花根部分离出的 I1R1 菌株的基因组采用 Pacbio Sequel 测序技术进行了全序列测序，组装成 8 条染色体，基因组大小为 30.8Mb，还有一个 26kb 的线粒体基因组。对 I1R1 基因组的结构和功能分析揭示了基因模型注释，其中编码了参与重要代谢过程的酶组，以及细胞色素 P450 和次生代谢物生物合成。基于同源性和基因家族复制的草酸青霉种的比较分析表明，它们具有 9500 个直系同源群的大型和封闭的泛基因组。这是未来系统发育和群体基因组学研究的有价值的数据。
