使用VecTest西尼罗河病毒抗原检测法快速检测鸟类中的西尼罗河病毒。
Rapid detection of West Nile Virus in birds using the VecTest WNV Antigen Assay.
作者信息
Henson Gay, Hicock Paul
机构信息
University of Mississippi Medical Center, Jackson, MS, USA.
出版信息
Clin Lab Sci. 2004 Fall;17(4):218-20.
OBJECTIVE
To determine if the VecTest West Nile Virus Antigen Assay (for testing mosquitoes) could be adapted to detect West Nile virus (WNV) rapidly and accurately in birds for screening purposes.
DESIGN
Cloacal swabs and tissue (kidney and spleen) were harvested from 40 fresh dead birds. The VecTest was used for each swab specimen for detection of WNV; PCR was used for each tissue specimen for confirmation of WNV.
SETTING
Mississippi Veterinary Diagnostic Laboratory (MVDL) in Jackson Mississippi and College of Veterinary Medicine-Mississippi State University (CVM-MSU) in Starkville Mississippi. SPECIMENS/SUBJECTS: Forty birds of the Corvid family (31 blue jays and 9 American crows) were included in the study. Fresh dead birds that died from no obvious cause were submitted for testing.
RESULTS
VecTest results were 35 positives and 5 negatives. PCR results were 35 positives and 5 negatives.
CONCLUSION
The VecTest showed 100% accuracy.
目的
确定VecTest西尼罗河病毒抗原检测法（用于检测蚊子）是否可适用于快速、准确地检测鸟类中的西尼罗河病毒（WNV），以用于筛查目的。
设计
从40只刚死亡的鸟类身上采集泄殖腔拭子和组织（肾脏和脾脏）。对每个拭子标本使用VecTest检测WNV；对每个组织标本使用PCR确认WNV。
地点
密西西比州杰克逊市的密西西比兽医诊断实验室（MVDL）和密西西比州立大学斯塔克维尔分校的兽医学院（CVM-MSU）。
标本/受试者：40只鸦科鸟类（31只冠蓝鸦和9只美洲鸦）被纳入研究。将无明显死因的刚死亡鸟类送检进行检测。
结果
VecTest检测结果为35个阳性和5个阴性。PCR检测结果为阳性35个，阴性5个。
结论
VecTest显示出100%的准确率。
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