Nemeth Nicole M, Beckett Susan, Edwards Eric, Klenk Kaci, Komar Nicholas
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA.
Am J Trop Med Hyg. 2007 Mar;76(3):431-7.
We tested 1,549 avian carcasses of 104 species to identify targets for West Nile virus (WNV) surveillance in Colorado, determine species affected by WNV, compare virus isolation versus RNA detection applied to hearts and oral swabs from carcasses, and compare the VecTest WNV Antigen Assay (VecTest) to standard assays. Forty-two species tested positive. From June to September 2003, 86% of corvids, 34% of non-corvid passerines, and 37% of raptors tested positive. We developed the Target Species Index, which identified American crows as the most important avian indicator species. However, testing multiple species maximizes detection, which may be important early and late in the transmission season. This index may benefit surveillance for other zoonotic pathogens, such as highly pathogenic avian influenza H5N1 virus. VecTest using oral swabs was most sensitive for American crow, black-billed magpie, house finch, house sparrow, and American kestrel. Wildlife rehabilitation centers should be recruited to enhance WNV surveillance.
我们检测了104种鸟类的1549具尸体，以确定科罗拉多州西尼罗河病毒（WNV）监测的目标，确定受WNV影响的物种，比较应用于尸体心脏和口腔拭子的病毒分离与RNA检测，并将VecTest WNV抗原检测法（VecTest）与标准检测法进行比较。42个物种检测呈阳性。在2003年6月至9月期间，86%的鸦科鸟类、34%的非鸦科雀形目鸟类和37%的猛禽检测呈阳性。我们制定了目标物种指数，该指数将美洲乌鸦确定为最重要的鸟类指示物种。然而，检测多个物种可使检测最大化，这在传播季节的早期和晚期可能很重要。该指数可能有益于对其他动物源性病原体的监测，例如高致病性禽流感H5N1病毒。使用口腔拭子的VecTest对美洲乌鸦、黑嘴喜鹊、家朱雀、家麻雀和美洲红隼最为敏感。应招募野生动物康复中心以加强WNV监测。
