[水通道蛋白-1及Bcl-2 mRNA在鼻息肉嗜酸性粒细胞中的表达及其意义]

[Expression of aquaporin-1 and Bcl-2 mRNA in eosinophils of the nasal polyps and its significance].

作者信息

Guan Gui-mei, Dong Zhen, Lü Mei

机构信息

Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031, China.

出版信息

Zhonghua Er Bi Yan Hou Ke Za Zhi. 2004 Aug;39(8):476-8.

PMID:15563082

Abstract

OBJECTIVE

To study the significance of aquaporin-1 (AQP-1) expression in the eosinophils of nasal polyps. The expression and location of AQP-1 mRNA and apoptosis associated gene Bcl-2 mRNA in nasal polyps were explored.

METHODS

Sixteen nasal polyp samples were collected from 11 women and 5 men aged 20-65 years during routine endonasal surgery. Nasal mucosa specimens from the inferior turbinates of 10 patients with allergic rhinitis (7 women and 3 men, aged 16-58 years), collected during septoplasty, were used as controls. The expression of AQP-1 mRNA and Bcl-2 mRNA was detected in serial adjacent sections by in situ hybridization and eosinophils were examined by stain MGG.

RESULTS

AQP-1 mRNA expression was found in all 16 nasal polyps and in 4 of 10 inferior turbinate tissues, the mean expression rates were (93.16 +/- 13.25)% and (19.54 +/- 4.98)%, respectively. All 16 nasal polyps and 10 control nasal tissues expressed Bcl-2 mRNA, by the average rates of (84.74 +/- 12.10)% and (16.45 +/- 3.12)%, respectively. The expression of AQP-1 mRNA was positively correlated with Bcl-2 mRNA expression in nasal polyps (r = 0.875, P < 0.01).

CONCLUSIONS

AQP-1 contributes to the survival of eosinophils in nasal polyps by keeping the permeation balance of eosinophils.

摘要

目的

研究水通道蛋白-1（AQP-1）在鼻息肉嗜酸性粒细胞中的表达意义。探讨AQP-1 mRNA和凋亡相关基因Bcl-2 mRNA在鼻息肉中的表达及定位。

方法

在常规鼻内镜手术中，收集了11名女性和5名男性（年龄20 - 65岁）的16份鼻息肉样本。将10例变应性鼻炎患者（7名女性和3名男性，年龄16 - 58岁）在鼻中隔成形术期间收集的下鼻甲鼻黏膜标本作为对照。通过原位杂交在连续相邻切片中检测AQP-1 mRNA和Bcl-2 mRNA的表达，并用MGG染色检查嗜酸性粒细胞。

结果

在所有16份鼻息肉以及10份下鼻甲组织中的4份中发现了AQP-1 mRNA表达，平均表达率分别为（93.16±13.25）%和（19.54±4.98）%。所有16份鼻息肉和10份对照鼻组织均表达Bcl-2 mRNA，平均表达率分别为（84.74±12.10）%和（16.45±3.12）%。鼻息肉中AQP-1 mRNA的表达与Bcl-2 mRNA的表达呈正相关（r = 0.875，P < 0.01）。