Zhang Junmei, Brodbelt Jennifer S
Department of Chemistry and Biochemistry, University of Texas, 1 University Station A5300, Austin, TX 78712, USA.
Analyst. 2004 Dec;129(12):1227-33. doi: 10.1039/b412577k. Epub 2004 Oct 26.
The main flavonoids in grapefruit juice, naringin and narirutin, were quantified by LC-MS with structural differentiation by LC-MS/MS. After human consumption of grapefruit juice, urine samples were collected for 24 hours and screened for flavonoid metabolites by LC-MS. The metabolite structures (glucuronides, sulfates, and glucuronide sulfates) were then confirmed via their unique fragmentation patterns by LC-MS/MS. To further verify the identity of the common aglycon (naringenin) shared by the metabolites, enzymatic hydrolysis was performed and the resulting products were analyzed. This work demonstrates that LC-MS and LC-MS/MS techniques can be used for fast metabolite screening without extensive sample preparation.
采用液相色谱-质谱联用（LC-MS）对葡萄柚汁中的主要黄酮类化合物柚皮苷和橙皮芸香苷进行定量，并通过液相色谱-串联质谱（LC-MS/MS）进行结构鉴别。人体饮用葡萄柚汁后，收集24小时尿液样本，并用LC-MS筛查黄酮类代谢物。然后通过LC-MS/MS独特的碎片模式确认代谢物结构（葡萄糖醛酸苷、硫酸盐和葡萄糖醛酸硫酸盐）。为进一步验证代谢物共有的常见苷元（柚皮素）的身份，进行了酶水解并分析了所得产物。这项工作表明，LC-MS和LC-MS/MS技术可用于快速代谢物筛查，无需进行大量样品制备。
