Zhang F, Chen G, Huang Q, Orion O, Krugman T, Fahima T, Korol A B, Nevo E, Gutterman Y
Institute of Forestry, Chinese Academy of Forestry, Haidian District, Beijing, 100091, China.
Theor Appl Genet. 2005 Feb;110(3):445-53. doi: 10.1007/s00122-004-1851-1. Epub 2004 Nov 24.
The genomic regions controlling caryopsis dormancy and seedling desiccation tolerance were identified using 152 F4 lines derived from a cross between Mona, a Swedish cultivar, and an Israeli xeric wild barley Hordeum spontaneum genotype collected at Wadi Qilt, Israel. Dormancy, the inability of a viable seed to germinate, and desiccation tolerance, the ability of the desiccated seedlings to revive after rehydration, were characterized by fitting the germination and revival data with growth curves, using three parameters: minimum, maximum, and slope of germination or revival rate derived by the least square method. The genetic map was constructed with 85 genetic markers (SSRs, AFLPs, STSs, and Dhn genes) using the MULTIPOINT: -mapping algorithm. Quantitative trait loci (QTLs) mapping was conducted with the MULTIQTL: package. Ten genomic regions were detected that affected the target traits, seven of which affected both dormancy and desiccation tolerance traits. Both the wild barley genotype and the Swedish cultivar contributed the favorite alleles for caryopsis dormancy, whereas seedling desiccation tolerance was attributed to alleles descending from the cultivar. The results indicate that some barley dormancy genes are lost during domestication and that dormancy QTLs are associated with abiotic stress tolerance.
利用152个F4株系鉴定了控制颖果休眠和幼苗脱水耐受性的基因组区域，这些株系来源于瑞典品种莫纳（Mona）与在以色列基尔特河谷（Wadi Qilt）采集的以色列旱生野生大麦（Hordeum spontaneum）基因型的杂交后代。休眠是指有活力的种子不能发芽的现象，脱水耐受性是指脱水后的幼苗在复水后能够复苏的能力，通过将发芽和复苏数据与生长曲线拟合来表征，使用三个参数：通过最小二乘法得出的发芽或复苏率的最小值、最大值和斜率。利用MULTIPOINT: -mapping算法，用85个遗传标记（SSR、AFLP、STS和Dhn基因）构建了遗传图谱。使用MULTIQTL: 软件包进行数量性状位点（QTL）定位。检测到10个影响目标性状的基因组区域，其中7个同时影响休眠和脱水耐受性性状。野生大麦基因型和瑞典品种都为颖果休眠贡献了有利等位基因，而幼苗脱水耐受性则归因于来自该品种的等位基因。结果表明，一些大麦休眠基因在驯化过程中丢失，并且休眠QTL与非生物胁迫耐受性相关。
