Institute of Crop Science, NARO, Tsukuba, Ibaraki, Japan.
Institute of Plant Science & Resources, Okayama University, Kurashiki, Japan.
Methods Mol Biol. 2024;2830:107-120. doi: 10.1007/978-1-0716-3965-8_10.
Seed dormancy is an important agronomic trait in cereal crops. Throughout the domestication of cereals, seed dormancy has been reduced to obtain uniform germination. However, grain crops must retain moderate levels of seed dormancy to prevent problems such as preharvest sprouting in wheat (Triticum aestivum) and barley (Hordeum vulgare). To produce modern cultivars with the appropriate seed dormancy levels, it is important to identify the genes responsible for seed dormancy. With recent advances in sequencing technology, several causal genes for seed dormancy quantitative trait loci (QTLs) have been identified in barley and wheat. Here, we present a method to identify causal genes for seed dormancy QTLs in barley, a method that is also applicable to other cereals.
种子休眠是谷类作物的一个重要农艺性状。在谷类作物的驯化过程中，为了获得整齐一致的发芽，种子休眠特性逐渐减弱。然而，粮食作物必须保持适度的种子休眠来防止一些问题，例如小麦（Triticum aestivum）和大麦（Hordeum vulgare）的穗发芽。为了生产具有适当休眠水平的现代品种，鉴定与种子休眠相关的基因非常重要。随着测序技术的最新进展，已在大麦和小麦中鉴定到几个与种子休眠数量性状位点（QTLs）相关的基因。在这里，我们提出了一种鉴定大麦种子休眠 QTL 相关基因的方法，该方法也适用于其他谷物。
