Ludwig Martin, Kohler Frank, Belder Detlev
Abteilung für Chromatographie, Max-Planck-Institut für Kohlenforschung, Mülheim an der Ruhr, Germany.
Electrophoresis. 2003 Sep;24(18):3233-8. doi: 10.1002/elps.200305427.
Fast chiral separations of a variety of basic and acidic compounds could be realized on microfluidic quartz chips. A microchip electrophoresis instrument equipped with a linear imaging UV-detector was used. The usually applied but troublesome fluorescence tagging in order to enable fluorescence detection could be omitted. Using sulfated cyclodextrins as chiral selectors baseline separation of 19 compounds could be achieved in less than 1 min with high reproducibility. The relative standard deviation of migration time was below 7%. The fastest separation could be performed in 2.5 s which is to date the fastest separation of enantiomers reported. It was possible to apply microchip electrophoresis (MCE) for the determination of high enantiomeric excess (ee) values, as exemplarily shown for pseudoephedrin where 2% of the minor enantiomer could reliably be determined beside high amount of the other isomer. Successful separation of a mixture of 3 chiral drugs could be performed in a single run in less than 11 s utilizing a separation length of only 12 mm. These results show that MCE has great potential for fast chiral analysis and high-throughput screening.
在微流控石英芯片上可以实现多种碱性和酸性化合物的快速手性分离。使用了配备线性成像紫外检测器的微芯片电泳仪。为实现荧光检测而通常采用但麻烦的荧光标记可以省去。以硫酸化环糊精作为手性选择剂，19种化合物可在不到1分钟内实现基线分离，且重现性高。迁移时间的相对标准偏差低于7%。最快的分离可在2.5秒内完成，这是迄今为止报道的对映体最快分离速度。微芯片电泳（MCE）可用于测定高对映体过量（ee）值，以伪麻黄碱为例，在存在大量其他异构体的情况下，仍能可靠地测定2%的次要对映体。利用仅12毫米的分离长度，在不到11秒的单次运行中就可以成功分离3种手性药物的混合物。这些结果表明，MCE在快速手性分析和高通量筛选方面具有巨大潜力。
