Rötting Anna K, Freeman David E, Constable Peter D, Eurell Jo Ann C, Wallig Matthew A
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
Am J Vet Res. 2004 Nov;65(11):1589-95. doi: 10.2460/ajvr.2004.65.1589.
To study the effects of phenylbutazone, indomethacin, prostaglandin E2 (PGE2), glutamine, and butyrate on restitution of oxidant-injured right dorsal colon of horses in vitro.
Right dorsal colon from 9 adult horses euthanatized for reasons other than gastrointestinal tract disease.
Mucosal segments from the right dorsal colon were injured via exposure to HOCl and incubated in Ussing chambers in solutions containing phenylbutazone, indomethacin, indomethacin and PGE2, glutamine, and butyrate. Transepithelial resistance and mucosal permeability to mannitol were measured, and all mucosal segments were examined histologically.
The HOCl-injured mucosa had lower resistance and higher permeability to mannitol, compared with control tissue. Histologic changes were also evident. Resistance of HOCl-injured mucosa recovered partially during the incubation period, and glutamine improved recovery. Phenylbutazone and indomethacin increased resistance, but these increases were not significant. Butyrate and PGE2 had no effects, compared with nontreated HOCl-injured tissues. Mucosal permeability to mannitol was lower in glutamine-treated tissue, compared with nontreated tissue. Histologic changes reflected the resistance and permeability changes.
According to our findings, phenylbutazone and indomethacin do not seem to interfere with restitution of oxidant-injured mucosa of equine colon in vitro, and glutamine could facilitate mucosal restitution.
研究保泰松、吲哚美辛、前列腺素E2（PGE2）、谷氨酰胺和丁酸盐对体外培养的马氧化损伤右背侧结肠修复的影响。
取自9匹因胃肠道疾病以外的原因实施安乐死的成年马的右背侧结肠。
将右背侧结肠的黏膜段暴露于次氯酸中造成损伤，然后置于Ussing小室中，在含有保泰松、吲哚美辛、吲哚美辛与PGE2、谷氨酰胺和丁酸盐的溶液中孵育。测量跨上皮电阻和黏膜对甘露醇的通透性，并对所有黏膜段进行组织学检查。
与对照组织相比，次氯酸损伤的黏膜对甘露醇的电阻较低且通透性较高。组织学变化也很明显。次氯酸损伤的黏膜电阻在孵育期部分恢复，谷氨酰胺可促进恢复。保泰松和吲哚美辛可增加电阻，但这些增加并不显著。与未处理的次氯酸损伤组织相比，丁酸盐和PGE2没有作用。与未处理的组织相比，谷氨酰胺处理的组织对甘露醇的黏膜通透性较低。组织学变化反映了电阻和通透性的变化。
根据我们的研究结果，保泰松和吲哚美辛似乎不会干扰体外培养的马结肠氧化损伤黏膜的修复，谷氨酰胺可促进黏膜修复。
