Lafeber F P, van Roy H, Wilbrink B, Huber-Bruning O, Bijlsma J W
Department of Rheumatology, University Hospital Utrecht, The Netherlands.
J Rheumatol. 1992 Jan;19(1):123-9.
The proteoglycan turnover of human osteoarthritic (OA) cartilage was compared to that of normal (N) cartilage. The cartilage was obtained postmortem from human femoral knee condyles. Short term cultures were compared to longterm cultures, and proteoglycan synthesis rate, content and release determined. Proteoglycan synthesis rate, determined shortly after collection of the cartilage, was higher in OA cartilage than in N cartilage. After longterm culture, the initially higher proteoglycan synthesis rate of OA cartilage became lower than that of N cartilage. An increased percentage release of proteoglycans from OA cartilage compared to N cartilage was apparent both shortly after collection of the cartilage and after culture. Thus, although OA cartilage in vivo is synthetically more active, in vitro it has an increased catabolic and a decreased anabolic activity, from which we conclude that OA cartilage is less vital than N cartilage.
将人类骨关节炎（OA）软骨的蛋白聚糖周转率与正常（N）软骨进行了比较。软骨取自人类股骨髁的尸体解剖。对短期培养和长期培养进行了比较，并测定了蛋白聚糖的合成速率、含量和释放量。在软骨采集后不久测定的蛋白聚糖合成速率，OA软骨高于N软骨。长期培养后，OA软骨最初较高的蛋白聚糖合成速率变得低于N软骨。与N软骨相比，无论是在软骨采集后不久还是培养后，OA软骨中蛋白聚糖的释放百分比都明显增加。因此，尽管OA软骨在体内的合成活性更高，但在体外其分解代谢活性增加而合成代谢活性降低，由此我们得出结论，OA软骨的活力低于N软骨。
