Ihara T, Yamawaki K, Fujiwara T, Kitamura K, Sakurai M, Kamiya H
Department of Pediatrics, Mie National Hospital, Japan.
Acta Paediatr Jpn. 1994 Jun;36(3):244-9. doi: 10.1111/j.1442-200x.1994.tb03172.x.
The generation of hydrogen peroxide (H2O2), one of the reactive oxygen species, by polymorphonuclear leukocytes (PMN) stimulated by Staphylococcus aureus and Escherichia coli was studied in infants by cytofluorography. After heparinized whole blood was incubated with bacteria for 60 min, generated H2O2 was measured. The positive rate of H2O2 generation of PMN and mean fluorescent intensity of positive PMN stimulated by S. aureus and E. coli were significantly reduced in infants aged < 1 year and H2O2 generation increased with advancing age. In 10-15 year old children, the level of generated H2O2 reached adult levels. When sera from 1 year old children were added to separated PMN from healthy adults, H2O2 generation was reduced. In contrast, H2O2 generation by PMN from 1 year old children was increased by the addition of adult sera. These results suggest that the ability to generate H2O2 in response to S. aureus and E. coli is lower in infants and that such reduced activity may be related to the susceptibility of such infants to S. aureus and E. coli infections.
通过细胞荧光术研究了金黄色葡萄球菌和大肠杆菌刺激的多形核白细胞（PMN）产生活性氧之一的过氧化氢（H2O2）的情况。将肝素化全血与细菌孵育60分钟后，测量产生的H2O2。1岁以下婴儿中，由金黄色葡萄球菌和大肠杆菌刺激的PMN产生H2O2的阳性率及阳性PMN的平均荧光强度显著降低，且H2O2产生随年龄增长而增加。在10 - 15岁儿童中，产生的H2O2水平达到成人水平。当将1岁儿童的血清添加到健康成人分离出的PMN中时，H2O2产生减少。相反，添加成人血清会增加1岁儿童PMN产生H2O2的量。这些结果表明，婴儿对金黄色葡萄球菌和大肠杆菌产生H2O2的能力较低，且这种活性降低可能与这类婴儿对金黄色葡萄球菌和大肠杆菌感染的易感性有关。
