Huang Her-Hsiung, Ho Chun-Te, Lee Tzu-Hsin, Lee Tien-Ling, Liao Ko-Kaung, Chen Fang-Lung
Institute of Oral Materials Science, Chung Shan Medical University, No. 110, Sec.1, Chien-Kuo North Road, Taichung 402, Taiwan.
Biomol Eng. 2004 Nov;21(3-5):93-7. doi: 10.1016/j.bioeng.2004.05.001.
The effect of surface roughness of ground Ti on the initial adhesion of osteoblast-like U-2 OS cells was investigated in this study. Different numbers (#120, #600, and #1500) of SiC sandpaper and two Al2O3 polishing powder (0.3 and 1 microm) were used to prepare the metal specimens with varying degrees of surface roughness. Surface roughness (Ra) was measured by profilometry. Surface topography was observed using an atomic force microscope. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay was used to measure the optical density (OD) of specimens after 2 h of cell incubation. The OD value was analyzed by one-way ANOVA for analyzing the factor of surface roughness. Crystal violet staining technique was used to characterize the cell spreading. Results showed that the specimen of #1500 Ti (Ra: 0.15 microm) had the highest OD value. The specimens polished with 0.3 and 1 microm Al2O3 powder (Ra: 0.05 and 0.07 microm) exhibited the worst cell adhesion behavior. Contact guidance of cells could be observed on the rougher #600 and #120 specimens (Ra: 0.33 and 1.20 microm). This study concludes that the surface roughness (Ra: 0.05-1.20 microm) of ground Ti has a highly significant influence on the initial adhesion of osteoblast-like U-2 OS cells. The ground Ti with an Ra of 0.15 microm shows the optimal cell adhesion behavior with respect to either the rougher or smoother specimens.
本研究调查了磨削钛表面粗糙度对成骨样U-2 OS细胞初始黏附的影响。使用不同目数（#120、#600和#1500）的碳化硅砂纸以及两种氧化铝抛光粉（0.3和1微米）制备具有不同表面粗糙度程度的金属试样。通过轮廓仪测量表面粗糙度（Ra）。使用原子力显微镜观察表面形貌。采用MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐）法测量细胞孵育2小时后试样的光密度（OD）。通过单因素方差分析对OD值进行分析，以分析表面粗糙度因素。使用结晶紫染色技术表征细胞铺展情况。结果表明，#1500钛试样（Ra：0.15微米）的OD值最高。用0.3和1微米氧化铝粉末抛光的试样（Ra：0.05和0.07微米）表现出最差的细胞黏附行为。在较粗糙的#600和#120试样（Ra：0.33和1.20微米）上可观察到细胞的接触导向。本研究得出结论，磨削钛的表面粗糙度（Ra：0.05 - 1.20微米）对成骨样U-2 OS细胞的初始黏附具有高度显著影响。相对于较粗糙或较光滑的试样，Ra为0.15微米的磨削钛表现出最佳的细胞黏附行为。
