Francis Jayaraj A, Rumbeiha Wilson, Nair Muraleedharan G
Bioactive Natural Products and Phytoceuticals, Department of Horticulture and National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA.
Life Sci. 2004 Dec 24;76(6):671-83. doi: 10.1016/j.lfs.2004.10.001.
Easter lily (Lilium longiflorum) flowers have been used in traditional medicine for alleviating many ailments. However, the chemical basis of its bioactivity has not been investigated. We have determined bioactive components in Easter lily flowers using lipid peroxidation and cyclooxygenase enzyme inhibitory assays and found to be kaempferol (1), kaempferol glycosides (2, 3, 4, 8, 9 and 10), quercetin glycosides (5, 6 and 7), a regaloside (11), a chalcone (12) and a fatty acid fraction (13). The structures of compounds were determined by NMR, IR, UV/VIS and mass spectroscopic studies. Compound 1 showed the highest COX-1 inhibition (94.1%) followed by 3, 8 and 12 with 38.7, 30.8 and 32.4%, respectively. Only compound 1 inhibited COX-2 enzyme by 36.9% at 80 ppm. In lipid peroxidation inhibitory assay, kaempferol showed 37 and 100 % inhibitions at 1 and 10 ppm, respectively. At 10 ppm, more than 20% inhibition was observed for compounds 4, 7, 10, 11 and 12 and 53% for compound 3. The compounds reported in here are isolated for the first time from Easter lily flowers including novel compounds 10, 11 and 12. Our results suggest that kaempferol and quercetin flavonoids contributed to the anecdotal medicinal properties of Easter lily flowers.
复活节百合（Lilium longiflorum）花在传统医学中被用于缓解多种疾病。然而，其生物活性的化学基础尚未得到研究。我们通过脂质过氧化和环氧化酶抑制试验确定了复活节百合花中的生物活性成分，发现它们是山奈酚（1）、山奈酚糖苷（2、3、4、8、9和10）、槲皮素糖苷（5、6和7）、一种帝王百合苷（11）、一种查尔酮（12）和一个脂肪酸馏分（13）。通过核磁共振、红外、紫外/可见光谱和质谱研究确定了化合物的结构。化合物1表现出最高的COX-1抑制率（94.1%），其次是化合物3、8和12，抑制率分别为38.7%、30.8%和32.4%。只有化合物1在80 ppm时对COX-2酶的抑制率为36.9%。在脂质过氧化抑制试验中，山奈酚在1 ppm和10 ppm时的抑制率分别为37%和100%。在10 ppm时，化合物4、7、10、11和12的抑制率超过20%，化合物3的抑制率为53%。本文报道的化合物首次从复活节百合花中分离得到，包括新化合物10、11和12。我们的结果表明，山奈酚和槲皮素类黄酮促成了复活节百合花传闻中的药用特性。
